HPVomics

A therapeutic and epitome resource for Human Papillomaviruses

Efficient putative sgRNAs (CRISPR/Cas9 targets)

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S.No.	HPV type	Target region	sgRNA seuences	PAM	Strand(F/R)	Start	End	GC (%)	Predicted effeciency (%)
1	HPV16	E7	GATGAAATAGATGGTCCAGC	TGG	F	106	125	45	46.17
2	HPV16	E7	GCAAGTGTGACTCTACGCTT	CGG	F	176	195	50	42.52
3	HPV16	E7	TTATGGTTTCTGAGAACAGA	TGG	R	1	20	35	41.22
4	HPV16	E7	GCAATTAAATGACAGCTCAG	AGG	F	78	97	40	41.11
5	HPV18	E7	GGGTGTTCAGAAACAGCTGC	TGG	R	39	58	55	43.32
6	HPV31	E7	GATGTCATAGACAGTCCAGC	TGG	F	106	125	50	49.29
7	HPV33	E7	GGTGTTACAAGTGTGACAAC	AGG	R	106	125	45	47.14
8	HPV33	E7	GTTGACACATAAACGAACTG	TGG	R	85	104	40	46.06
9	HPV35	E7	ATGCTCTCTGTGAACAGCCG	GGG	R	3	22	55	58.39
10	HPV35	E7	GCAATTGTGTGACAGCTCAG	AGG	F	78	97	50	50.41
11	HPV35	E7	TGTTTTAGATTTGGAACCCG	AGG	F	33	52	40	43.4
12	HPV39	E7	GATGTTGGTGATTAACTGCA	TGG	R	180	199	40	51.45
13	HPV39	E7	TGGTTTGCAGTTGCACACCA	CGG	R	5	24	50	42.72
14	HPV45	E7	TATTGGTTAGTTGCACACCA	CGG	R	2	21	40	54.82
15	HPV45	E7	CCTTGTCCTTTGTGTGTCCG	TGG	F	281	300	55	44.55
16	HPV45	E7	ATTTCTTGCAGTGTTTCCCG	GGG	R	290	309	45	41.37
17	HPV51	E7	ACATCTGCTGTACAACGCGA	AGG	R	48	67	50	54.62
18	HPV52	E7	GCATTTGCTGTAGAGTACGA	AGG	R	48	67	45	54.97
19	HPV53	E7	TGAGCAATTGAACAGCTCAG	AGG	F	78	97	45	47.01
20	HPV53	E7	ACAAATGCTTATGGGCACAG	TGG	F	261	280	45	44.83
21	HPV56	E7	GGACATTCAGAGTACCAAAG	AGG	F	222	241	45	54.8
22	HPV56	E7	GCAGCTGTTGTACAACACGC	AGG	R	51	70	55	45.7
23	HPV56	E7	ACAAACTTACACTCACAACA	AGG	R	107	126	35	44.01
24	HPV58	E7	GTTGATACACAAACGAACCG	TGG	R	85	104	45	68.13
25	HPV58	E7	ATAGGCTTGGACGGGCCAGA	TGG	F	109	128	60	49.28
26	HPV59	E7	AATGAAAAAGATGAACCAGA	TGG	F	112	131	30	49.49
27	HPV59	E7	GGCTCGCAATCCGTCTTGCG	AGG	R	64	83	65	45.79
28	HPV59	E7	GTAGTAGAAACCTCGCAAGA	CGG	F	229	248	45	43.3
29	HPV66	E7	GGACATTCAGAGTACCAAAG	AGG	F	222	241	45	54.8
30	HPV68	E7	GTTGGTGGTGATTAACTGCA	TGG	R	183	202	45	49.71
31	HPV68	E7	TCTGTGTTGTAAGTGTAACA	AGG	F	201	220	35	49.39
32	HPV68	E7	TAACACAATTTCCTGCACGG	TGG	R	295	314	45	40.48
33	HPV73	E7	CGAGTCATTGGACAACTCAG	AGG	F	78	97	50	44.15
34	HPV82	E7	ACATTTGCTGAAATATGCGA	AGG	R	48	67	35	51.78
35	HPV82	E7	GCAATTTGACAGCTCAGACG	AGG	F	81	100	50	44.53
36	HPV82	E7	CAGCTCGCAGTGGAAAGCAG	TGG	F	205	224	60	42.66
37	HPV82	E7	GAGTGTTGTACAGCTCGCAG	TGG	F	195	214	55	41.09