| S.No. | HPV type | Target region | sgRNA seuences | PAM | Strand(F/R) | Start | End | GC (%) | Predicted effeciency (%) |
|---|---|---|---|---|---|---|---|---|---|
| 1 | HPV6 | E5 | TGCACTATAGAGCTGTACAA | GGG | F | 95 | 114 | 40 | 52.55 |
| 2 | HPV40 | E5 | GGATGATGGTGATACCTGGA | TGG | F | 18 | 37 | 50 | 48.64 |
| 3 | HPV40 | E5 | GGTATACCTATATAAATATA | AGG | R | 7 | 26 | 20 | 40.16 |
| 4 | HPV61 | E5 | GTTGTGGATCAGCAAAACTG | CGG | F | 46 | 65 | 45 | 43.69 |
| 5 | HPV72 | E5 | GTACTACGAGACGATAATTG | TGG | F | 46 | 65 | 40 | 42.24 |