HPVomics

A therapeutic and epitome resource for Human Papillomaviruses

Efficient putative sgRNAs (CRISPR/Cas9 targets)

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S.No.	HPV type	Target region	sgRNA seuences	PAM	Strand(F/R)	Start	End	GC (%)	Predicted effeciency (%)
1	HPV6	E6	ATATGCATATAAACACCTAA	AGG	F	135	154	25	62.46
2	HPV6	E6	TCATAAAGCTAAATTGTACG	TGG	F	377	396	30	52.9
3	HPV6	E6	CTAAAGGTCCTGTTTCGAGG	CGG	F	151	170	50	52.23
4	HPV6	E6	GGTAAAACATATACTAACCA	AGG	F	348	367	30	41.71
5	HPV11	E6	ATATGCCTATAAGAACCTAA	AGG	F	135	154	30	67.1
6	HPV11	E6	TCATAAAACTAAATAACCAG	TGG	F	377	396	25	52.68
7	HPV11	E6	ATATGCATATATCTCTGCGG	TGG	R	316	335	40	52.54
8	HPV11	E6	GGCATATGCATATATCTCTG	CGG	R	313	332	40	51.08
9	HPV11	E6	TATAGATGTTGCAGACGTGG	AGG	R	415	434	45	44.14
10	HPV40	E6	ATATAACTCTCTAAAGGCAA	AGG	R	313	332	30	48.46
11	HPV40	E6	CGCAGCCTATGCACCAACCG	TGG	F	243	262	65	48.42
12	HPV40	E6	CCACAAGCCTTTGTCTCCCG	TGG	F	318	337	60	45.1
13	HPV40	E6	ACATTGCTGGAAGAAATGCA	TGG	F	411	430	40	43.05
14	HPV42	E6	GCATTGCAGAGGACAATGCG	TGG	F	414	433	55	53.94
15	HPV42	E6	GGTCGGTGTACGCATTGCAG	AGG	F	403	422	60	44.04
16	HPV43	E6	GTAGCTGCTCCCAAAACGCA	CGG	F	11	30	55	61.76
17	HPV43	E6	TGCAAACGATAATACTTCCG	TGG	R	328	347	40	43.26
18	HPV43	E6	GGTGCATATTTTGCAAGAAG	TGG	F	89	108	40	41.3
19	HPV44	E6	CCACAAACCATTGTGCCACG	TGG	F	321	340	55	65.64
20	HPV44	E6	TGTGGCGCACCTTTTCCACG	TGG	F	96	115	60	58.27
21	HPV44	E6	CAGTTATATGTAGTGTACCG	AGG	F	148	167	40	46.85
22	HPV44	E6	ATTTAGGCATTTTAACTACG	CGG	F	228	247	30	44.8
23	HPV54	E6	GGAAGTTGCAAACTATGCAT	AGG	R	344	363	40	52.73
24	HPV54	E6	GCACTGTGCCTAGAACTGCA	CGG	F	286	305	55	52.45
25	HPV54	E6	TCCAATAGCCTCTAACACAG	TGG	R	33	52	45	49.03
26	HPV54	E6	CCATAAACCTTTGTGCAACG	TGG	F	414	433	45	48.83
27	HPV54	E6	AATCAGCTAGAGTACGCGGC	TGG	R	387	406	55	42.34
28	HPV61	E6	GGAAGGTGTTGCCAGTGTAG	AGG	F	391	410	55	53.87
29	HPV61	E6	GCTCTGCAAGGACTACGAGG	TGG	F	33	52	60	48.07
30	HPV61	E6	CCTGCTCTGCAAGGACTACG	AGG	F	30	49	60	47.82
31	HPV61	E6	GGCACGTGAAGTAAAAGTGA	GGG	F	192	211	45	45.19
32	HPV61	E6	GGAAAAGGAGCATCAGGTAC	AGG	F	330	349	50	44.78
33	HPV70	E6	CTGCTGGACCAGCAACCGGG	AGG	F	420	439	70	53.95
34	HPV70	E6	ATTTATAGTATATAGAAACG	GGG	F	156	175	20	51.03
35	HPV70	E6	AACACAGCTACAGCAAACAG	AGG	F	108	127	45	47.11
36	HPV72	E6	GGAAGGTGCTGCCAGTGTAG	AGG	F	397	416	60	53.11
37	HPV72	E6	GTTGGATTGTGCAGTCCCAT	AGG	R	422	441	50	44.41
38	HPV72	E6	GGAGCTGCAGATTGTGTGGC	GGG	F	141	160	60	43.93
39	HPV72	E6	CATGGTGCGCAGACTCCAAA	TGG	R	254	273	55	42.99
40	HPV72	E6	GCCTGTTTCCTGTTCCACAG	TGG	R	172	191	55	40.56
41	HPV81	E6	GGAAAGTGCTGCCATTGTAG	AGG	F	415	434	50	45.7
42	HPV81	E6	GTTTAGCTCTCTTATTGCAA	AGG	R	298	317	35	40.06