HPVomics

A therapeutic and epitome resource for Human Papillomaviruses

Efficient putative sgRNAs (CRISPR/Cas9 targets)

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S.No.	HPV type	Target region	sgRNA seuences	PAM	Strand(F/R)	Start	End	GC (%)	Predicted effeciency (%)
1	HPV16	E4	GGCTTTGGTATGGGTCGCGG	CGG	R	188	207	65	54.64
2	HPV16	E4	GCATTTAACAGCTCACACAA	AGG	F	231	250	40	48.36
3	HPV18	E4	GGTGCTGGAATACGGTGAGG	GGG	R	176	195	60	59.3
4	HPV18	E4	GGTGTAGCTGCACCGAGAAG	TGG	R	54	73	60	44.63
5	HPV18	E4	GTATTCCAGCACCGTGTCCG	TGG	F	80	99	60	43.47
6	HPV18	E4	CTACAGGCAACAACAAAAGA	CGG	F	214	233	40	41.86
7	HPV26	E4	CGTGGGCACCAAGGAAACCG	AGG	F	113	132	65	55.03
8	HPV26	E4	GGAAAGCGACGACGACTCAG	TGG	F	147	166	60	52.81
9	HPV26	E4	GTAGGCTTCGGTGGTCTGGG	TGG	R	215	234	65	49.29
10	HPV26	E4	CTACGTGCCCGTGGGCACCA	AGG	F	104	123	70	47.35
11	HPV26	E4	GGCTTCGGTGGTCTGGGTGG	TGG	R	218	237	70	45.99
12	HPV31	E4	GTTGTTGCGAGGCGACTCCG	TGG	F	203	222	65	54.6
13	HPV31	E4	GGTTTTGGAATTCGATGTGG	TGG	R	206	225	45	50.99
14	HPV31	E4	GCACCAGTGAAGGTGTGCGG	CGG	F	115	134	65	49.94
15	HPV33	E4	CTAACTGCACAAACAAGCAG	CGG	F	199	218	45	53.82
16	HPV33	E4	TGGTTGCTAGATATAGACGT	AGG	R	222	241	40	52.83
17	HPV35	E4	GCGAGTGCGACTCAGTGCCG	TGG	F	185	204	70	59.24
18	HPV35	E4	CCATACCAAAGCCTGCTCCG	TGG	F	89	108	60	51.51
19	HPV35	E4	GGCTTTGGTATGGGTCTCGG	TGG	R	191	210	60	50.19
20	HPV39	E4	GGACACTCCGTCGGGCTCAG	TGG	R	89	108	70	51.73
21	HPV39	E4	GTACAGGCCACAACACAAGA	CGG	F	232	251	50	47.44
22	HPV39	E4	ACAGAGCCCACTGAGCCCGA	CGG	F	168	187	65	44.6
23	HPV39	E4	GGATTGTTTTTTGGGTGCAT	GGG	R	157	176	40	42.89
24	HPV39	E4	GGTGCATGGGGTTGTTGCGG	TGG	R	170	189	65	41.48
25	HPV45	E4	GGACTCACAGAGCAGCACCA	CGG	F	156	175	60	78.02
26	HPV45	E4	GTACAAGTAACAACAAAAGA	AGG	F	220	239	30	52.34
27	HPV45	E4	CGTGGGTGTTGACACGTCCG	TGG	R	79	98	65	47.3
28	HPV45	E4	TGTTACTTGTACTTGAACAC	AGG	F	43	62	35	42.16
29	HPV51	E4	GGAAGTGGAAGCACTAACAC	TGG	F	207	226	50	58.58
30	HPV51	E4	GGACAGTGGGGAGATTGTGG	AGG	R	89	108	60	45.6
31	HPV51	E4	ACAAACAACCAAATACACTG	TGG	F	186	205	35	44.58
32	HPV51	E4	CACAATCTCCCCACTGTCCG	TGG	F	158	177	60	41.12
33	HPV51	E4	TTTGTATTGTCCACGGACAG	TGG	R	75	94	45	40.48
34	HPV52	E4	GGACACTGCGGAGGTCTTGG	AGG	R	200	219	65	50.83
35	HPV52	E4	GGCACCCACGGACACTGCGG	AGG	R	191	210	75	46.81
36	HPV52	E4	TTTGCGGGGACAACAATCCG	TGG	F	188	207	55	46.54
37	HPV52	E4	GGTCGTCGTCGTTTCTGTGG	TGG	R	150	169	60	44.22
38	HPV53	E4	GGACTCTCTGGCTGTGGTAG	TGG	R	122	141	60	60.09
39	HPV53	E4	GGGACGTTTTCCCGGCCGCA	GGG	R	185	204	70	58.07
40	HPV53	E4	TTATACAAACTTTAAAGACG	AGG	F	41	60	25	55.22
41	HPV53	E4	GGAGGTGGTGGGGGTCTTAT	GGG	R	239	258	60	54.72
42	HPV53	E4	GGCTGTGGTAGTGGACTGTG	TGG	R	131	150	60	52.52
43	HPV53	E4	ATATGGGTGTAAAGGCACAT	GGG	F	71	90	40	51.8
44	HPV53	E4	AACAACACCGAAATCGACAG	TGG	F	411	430	45	48.73
45	HPV53	E4	GGCACATGGGAAGTGCATAT	GGG	F	84	103	50	45.69
46	HPV53	E4	GGTGCCCACGGGCGTGGAGG	TGG	R	224	243	80	44.75
47	HPV53	E4	TGTGGTAGTGGACTGTGTGG	TGG	R	134	153	55	43.15
48	HPV53	E4	GTAGGTGCCCACGGGCGTGG	AGG	R	221	240	75	41.17
49	HPV56	E4	GGACGTGGAGGTGGTGGTGG	TGG	R	200	219	70	56.67
50	HPV56	E4	ATTTGGGTGTAAAAACATAT	GGG	F	56	75	25	43.49
51	HPV56	E4	GGTGGTGGTGGTGGTCTTGT	GGG	R	209	228	60	42.36
52	HPV58	E4	CCAACAACGAAAGTACACAG	GGG	F	76	95	45	59.61
53	HPV58	E4	GCCGTGGACAGTAGACCACG	AGG	F	171	190	65	51.17
54	HPV58	E4	TGCTGACCCAAAGACCACCG	AGG	F	50	69	60	50
55	HPV58	E4	AAAGTATACAGACTGCGCCG	TGG	F	155	174	50	47.37
56	HPV58	E4	CTAACTGTACATACAAAGGG	CGG	F	214	233	40	42.73
57	HPV59	E4	GGAAGTGCATTATAATGGCA	AGG	F	35	54	40	57.76
58	HPV59	E4	GGAAAGCGACCAAGACAGTG	TGG	F	204	223	55	50.37
59	HPV59	E4	ATAGGCGTAGTGTTACTGCA	AGG	R	3	22	45	47.03
60	HPV59	E4	TTGCTCAGAAGATCCAGCAG	TGG	R	221	240	50	43.68
61	HPV59	E4	AATATGGGACTACAGACAAG	TGG	F	13	32	40	40.6
62	HPV66	E4	TGTGGGCGCCCAAGACGCCG	CGG	F	320	339	75	51.41
63	HPV66	E4	TCCTGGTCTGTGGGATACCG	CGG	R	155	174	60	47.42
64	HPV66	E4	ATATGGGTGTACAAACATAT	GGG	F	167	186	30	46.36
65	HPV66	E4	TTACACAGACTTTGAACAGG	AGG	F	137	156	40	42.52
66	HPV66	E4	GGGTGGTGTAAAGTGTCATC	AGG	F	66	85	50	42.44
67	HPV66	E4	GGCGTCTTGGGCGCCCACAA	AGG	R	176	195	70	41.85
68	HPV66	E4	TGGCTTTGTGTGTTGATACG	TGG	R	27	46	45	41.14
69	HPV68	E4	GGACACGTTGTCGGGCTCAG	AGG	R	89	108	65	49.42
70	HPV68	E4	GGTGCATGGGGCACCTGCGG	TGG	R	170	189	75	47.65
71	HPV68	E4	TGAGCCCGACAACGTGTCCG	TGG	F	179	198	65	42.42
72	HPV68	E4	TGGGAGGTCGACACCGTCCA	CGG	R	68	87	65	41.86
73	HPV73	E4	GGTGTCAACGTTGGTGCAGG	TGG	R	128	147	60	53.45
74	HPV73	E4	GGTGGTGGTGTTGGTGGTTG	TGG	R	149	168	60	50.36
75	HPV73	E4	GGTGCAGGTGGTGGTGGTGT	TGG	R	140	159	65	42.12
76	HPV73	E4	AGGTTATGCAAACAATCCAG	GGG	R	57	76	40	41.34
77	HPV82	E4	GCAGGTGTCCACGGACAGCG	GGG	R	77	96	70	66.8
78	HPV82	E4	GGAATAGGTATTGGTCGTGG	GGG	R	182	201	50	57.11
79	HPV82	E4	CTGCAGGCCCAAACACCGGA	GGG	F	214	233	65	46.18
80	HPV82	E4	GGACAGCGGGGTGACTGTGG	AGG	R	89	108	70	46.14
81	HPV82	E4	GGGGTGACTGTGGAGGAGTC	GGG	R	96	115	65	44.55
82	HPV82	E4	GGCGGAATAGGTATTGGTCG	TGG	R	179	198	55	44.05
83	HPV82	E4	GGCTTCTGTGGTGCCCGCGG	AGG	R	158	177	75	43.72
84	HPV82	E4	GGAACTGCAGGCCCAAACAC	CGG	F	210	229	60	41.43