HPVomics

A therapeutic and epitome resource for Human Papillomaviruses

Efficient putative sgRNAs (CRISPR/Cas9 targets)

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S.No.	HPV type	Target region	sgRNA seuences	PAM	Strand(F/R)	Start	End	GC (%)	Predicted effeciency (%)
1	HPV16	E6	TTATGCATAGTATATAGAGA	TGG	F	169	188	25	47.44
2	HPV16	E6	CTAATTAACAAATCACACAA	CGG	R	152	171	25	45.04
3	HPV18	E6	GCACAGATCAGGTAGCTTGT	AGG	R	424	443	50	40.46
4	HPV26	E6	GGTACAGTGTGTATATTGCA	AGG	F	81	100	40	47.12
5	HPV31	E6	TATAGGGACGACACACCACA	CGG	F	160	179	50	55.16
6	HPV31	E6	CATTTTGTACACACTCCGTG	TGG	R	254	273	45	54.3
7	HPV31	E6	TTAGAAAAATTGACAAACAA	AGG	F	262	281	20	48.22
8	HPV31	E6	GCATGAACTAAGCTCGGCAT	TGG	F	36	55	50	42.35
9	HPV33	E6	CACAGTTTACATATTCCAAA	TGG	R	254	273	30	46.33
10	HPV33	E6	GCATGATTTGTGCCAAGCAT	TGG	F	36	55	45	41.74
11	HPV33	E6	TTTTTTTCTTGAGGACACAA	AGG	R	104	123	30	40.15
12	HPV35	E6	ACCAACACGTAGAGAAACCG	AGG	F	423	442	50	54
13	HPV35	E6	ACTGCAAACAAGAATTACAG	CGG	F	95	114	35	52.62
14	HPV35	E6	ACTGCATGATTTGTGCAACG	AGG	F	33	52	45	50.89
15	HPV35	E6	TCTACGTGTTGGTTTCCAAC	AGG	R	16	35	45	43.44
16	HPV39	E6	ATATGTAGTATATAGGGACG	GGG	F	156	175	35	56.22
17	HPV39	E6	ACGACCACTACAGCAAACCG	AGG	F	108	127	55	54.68
18	HPV39	E6	TTATATGTAGTATATAGGGA	CGG	F	154	173	25	49.66
19	HPV39	E6	GTGCTGGACCACAAAACGGG	AGG	F	420	439	60	43.06
20	HPV45	E6	AGCATAGCTGGACAGTACCG	AGG	F	385	404	55	56.78
21	HPV51	E6	ATAGATGTCAAAGACCACTT	GGG	F	311	330	35	40.07
22	HPV53	E6	TATAGAGACGGGTATCCGTA	TGG	F	172	191	45	47.39
23	HPV53	E6	CTAAGAGTAGTGTATAGAGA	CGG	F	160	179	35	42.64
24	HPV53	E6	TCAACATCCGTTGACACCAG	AGG	F	330	349	50	40.82
25	HPV58	E6	TTAAGAATAGTGTATAGAGA	TGG	F	148	167	25	43.74
26	HPV58	E6	GGCATGTGGATTTAAACAAA	AGG	F	350	369	35	41.12
27	HPV59	E6	ATATATACAGGACAGTGTCG	TGG	F	397	416	40	51.85
28	HPV59	E6	TTGATGCGAATATCATGCAG	AGG	R	392	411	40	41.54
29	HPV66	E6	GATAATCTAAGATCAAGTAA	AGG	R	374	393	25	44.92
30	HPV66	E6	TCAATGTCCGTTAACACCGG	AGG	F	327	346	50	40.15
31	HPV68	E6	GGCGCTATTTCACAACCCTG	AGG	F	3	22	55	51.64
32	HPV68	E6	ATGTGTAGTGTATAGAGACG	GGG	F	156	175	40	50.9
33	HPV68	E6	CTATGTGTAGTGTATAGAGA	CGG	F	154	173	35	50.62
34	HPV68	E6	CTGCTGGACCAGTAAGCGAG	AGG	F	420	439	60	40.29
35	HPV73	E6	TTCATCAAATAGCAGAACAG	TGG	F	377	396	35	43.21
36	HPV82	E6	CTAGAATTAGAGAATACAGA	AGG	F	212	231	30	60.29
37	HPV82	E6	GCCTGAAGAAAAGCAAAAGG	TGG	F	333	352	45	47.39
38	HPV82	E6	ATAGATGTCAGAGACCACTT	GGG	F	311	330	40	43.33