HPVomics

A therapeutic and epitome resource for Human Papillomaviruses

Efficient putative sgRNAs (CRISPR/Cas9 targets)

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S.No.	HPV type	Target region	sgRNA seuences	PAM	Strand(F/R)	Start	End	GC (%)	Predicted effeciency (%)
1	HPV6	L1	GTTCCAGTCTTCCAAAACAG	AGG	R	310	329	45	54.1
2	HPV6	L1	TATTGGCTACAAAAAGCCCA	GGG	F	919	938	40	51.02
3	HPV6	L1	ATATATGTTAACACCCCGAG	CGG	F	856	875	40	49.71
4	HPV6	L1	GGGTTATCGCCTCCCCCAAA	TGG	F	1198	1217	60	48.91
5	HPV6	L1	GGCATGCACAGGCCTAGAGG	TGG	F	291	310	65	48.76
6	HPV6	L1	GGGATTCATTGTGTGAATAT	AGG	R	331	350	35	47.81
7	HPV6	L1	GGTGTCAGGATATCAATACA	GGG	F	183	202	40	47.79
8	HPV6	L1	ACAGGCCTAGAGGTGGGCAG	GGG	F	298	317	65	46.91
9	HPV6	L1	GTACATTAGAAGATACCTAT	AGG	F	1220	1239	30	46.83
10	HPV6	L1	GTAAGCGCGCCAAAACTAAA	AGG	R	1478	1497	45	46.34
11	HPV6	L1	GTATCTTCTAATGTACCATT	TGG	R	269	288	30	45.15
12	HPV6	L1	ATATTGGCTACAAAAAGCCC	AGG	F	918	937	40	44.05
13	HPV6	L1	GGACGGTCCTCTATTCGTAC	AGG	R	1411	1430	55	42.38
14	HPV6	L1	GGATGCACATAATGTCATGT	TGG	F	475	494	40	41.7
15	HPV6	L1	GGTTGGATGTGCCCCCCCTT	TGG	F	462	481	65	41.67
16	HPV6	L1	CCAATGCTCGCCCAAAGGGG	GGG	R	1009	1028	65	41.49
17	HPV6	L1	GGACACCAAAGAGCCGCTCG	GGG	R	613	632	65	40.91
18	HPV6	L1	TTACCCCAATGCTCGCCCAA	AGG	R	1004	1023	55	40.48
19	HPV6	L1	CCACTTACACCCACACCTAA	TGG	R	1157	1176	50	40.47
20	HPV6	L1	ATATCCAGATTATTTACAAA	TGG	F	678	697	20	40.22
21	HPV11	L1	GTAAATCAAACTCCTCCACA	TGG	R	402	421	40	56.86
22	HPV11	L1	GCAGTCACCATTTTGTACAG	AGG	R	961	980	45	56.49
23	HPV11	L1	GATGATGTAGAAAATAGTGG	TGG	F	370	389	35	54.52
24	HPV11	L1	GATGACCTGTTGGTAAAAGG	GGG	F	808	827	45	50.8
25	HPV11	L1	GTATCCTCCAGTGTACCATT	TGG	R	269	288	45	50.49
26	HPV11	L1	GGTTTATCGCCTCCACCAAA	TGG	F	1201	1220	50	49.83
27	HPV11	L1	CTACCACAGTAACAAACAAG	TGG	R	513	532	40	49.48
28	HPV11	L1	ACAGGGTTGGAGGTAGGCAG	GGG	F	298	317	60	47.26
29	HPV11	L1	ACAGTTCCACAAATATCAAG	GGG	R	833	852	35	46.66
30	HPV11	L1	GGTTTTGGTACGTTTTCGTT	TGG	R	10	29	40	45.61
31	HPV11	L1	ACTGGTAATAAGTTCCAACG	GGG	R	937	956	40	44.49
32	HPV11	L1	ACACTGGTAATAAGTTCCAA	CGG	R	935	954	35	44.14
33	HPV11	L1	TCCAGTGTACCATTTGGTGG	AGG	R	275	294	50	43.47
34	HPV11	L1	GGAATACATGCGCCATGTGG	AGG	F	1071	1090	55	43.39
35	HPV11	L1	ATTTATGTACATACACCTAG	TGG	F	859	878	30	42.59
36	HPV11	L1	GCGTTTAACATACGCATCCG	TGG	R	1420	1439	50	41.15
37	HPV40	L1	GGAATGGCTGCAGAACCGTA	TGG	F	700	719	55	66.09
38	HPV40	L1	GGTTGGTGTGGGGGACTGTG	TGG	R	463	482	65	61.15
39	HPV40	L1	TTTGAGTTAAAAAAACCCAA	TGG	F	145	164	25	60.55
40	HPV40	L1	ATATGGATCCTCCCGTACCT	TGG	R	199	218	50	53.87
41	HPV40	L1	CTAATACAGGACAGTCCCCA	AGG	R	960	979	50	53.27
42	HPV40	L1	GGCACTGAAATGTCACCATT	GGG	R	1337	1356	45	48.69
43	HPV40	L1	GGGGGTGACTGGCTTAGAAG	AGG	R	25	44	60	47.73
44	HPV40	L1	GGTTAAATTAGTGCTACGAG	TGG	R	496	515	40	46.81
45	HPV40	L1	GGATCCTCCCGTACCTTGGG	GGG	R	203	222	65	46.55
46	HPV40	L1	GTAGCAACAGGCGTTGGCGG	TGG	R	1463	1482	65	46.44
47	HPV40	L1	ATACTCATCTGTGCTAACAA	TGG	R	1441	1460	35	46.25
48	HPV40	L1	GGCCACTAACACCAACCCCT	AGG	R	1167	1186	60	44.8
49	HPV40	L1	TGGCTTAGAAGAGGAAGACG	AGG	R	34	53	50	44.61
50	HPV40	L1	ATAAGGAACTTACGTCCTAA	TGG	R	113	132	35	43.12
51	HPV40	L1	ATGCTTCTCGGGTAACCCTT	GGG	F	521	540	50	43.1
52	HPV40	L1	GTTACCCGAGAAGCATTGCA	CGG	R	983	1002	50	42.9
53	HPV40	L1	ATAGATGTGCATAAATCCAA	TGG	R	842	861	30	42.79
54	HPV40	L1	TTACTGTTATTATATGGGGT	TGG	R	446	465	30	42.53
55	HPV40	L1	CGTTTCAGAATTAAACAAGG	AGG	R	1240	1259	35	42.05
56	HPV40	L1	TATGACATATATTCATGCAA	TGG	F	1158	1177	25	41.73
57	HPV40	L1	GGGCTGCACACCCCCAATTG	GGG	F	471	490	65	41.56
58	HPV40	L1	CCACTAACACCAACCCCTAG	GGG	R	1169	1188	55	41
59	HPV40	L1	GGAATATTTGCGTCATGGGG	AGG	F	1080	1099	50	40.55
60	HPV40	L1	GTGGGTGTGGAGGTCGGCCG	TGG	F	301	320	75	40.13
61	HPV42	L1	CCACTAATACCAACGCCCAA	AGG	R	1157	1176	50	54.46
62	HPV42	L1	GTTGTCACTAGGCCGCCACA	CGG	R	1483	1502	60	53.78
63	HPV42	L1	ACTGTCTGTAGGTAAACGAA	AGG	F	1434	1453	40	52.4
64	HPV42	L1	GATGTTACCATAGAACCACT	AGG	R	605	624	40	52.37
65	HPV42	L1	TATTGGTTACAACAAGCACA	AGG	F	931	950	35	50.92
66	HPV42	L1	GTGGGGCTAGAAGTAGGTCG	TGG	F	304	323	60	50.58
67	HPV42	L1	CTATCTTCTAAAGTTCCTGA	AGG	R	263	282	35	49.32
68	HPV42	L1	GGGCAGTCACCATTGGACTG	TGG	R	962	981	60	47.62
69	HPV42	L1	ACCACCTTGGAAACAGGAGG	AGG	R	1445	1464	55	42.3
70	HPV42	L1	CCTGCCATAGGGGAGCACTG	GGG	F	484	503	65	41.46
71	HPV42	L1	GTAGGTCGTGGACAGCCTTT	GGG	F	316	335	55	41.19
72	HPV42	L1	CTGACCACCTTGGAAACAGG	AGG	R	1442	1461	55	40.89
73	HPV43	L1	GGGCACAGTAGGGTCAGTAG	AGG	R	460	479	60	59.91
74	HPV43	L1	GGCAGGGGCAGACGTAGAGG	AGG	R	28	47	70	55.85
75	HPV43	L1	GTAGGAGTTGAAATTGGTAG	AGG	F	301	320	40	55.6
76	HPV43	L1	GGGGCAGACGTAGAGGAGGA	TGG	F	32	51	65	55.59
77	HPV43	L1	GGCGATGTGCCATACCCAGA	CGG	R	1103	1122	60	54.18
78	HPV43	L1	GGATGCGTAGGAGTTGAAAT	TGG	F	295	314	45	52.56
79	HPV43	L1	CCAGCAATATACATATCGGA	AGG	R	683	702	40	51.87
80	HPV43	L1	GATGACACTGAAAACCCGTC	TGG	F	373	392	50	51.69
81	HPV43	L1	AGTATCTTCCAAAGAAGCAG	AGG	R	265	284	40	50.89
82	HPV43	L1	ATATATTTTTCTACACCCAG	TGG	F	862	881	30	50.77
83	HPV43	L1	TTATGGATACAAAAGGCCCA	GGG	F	925	944	40	47.49
84	HPV43	L1	CTATTACAGGACAATCACCT	TGG	R	954	973	40	46.22
85	HPV43	L1	GTAACACCTGATGCGTTGCA	AGG	R	977	996	50	45.18
86	HPV43	L1	GGGATCCTCCCGTTCTTTTG	GGG	R	196	215	55	41.86
87	HPV43	L1	GCCAGCATAATAAAATAAGT	TGG	R	1402	1421	30	41.77
88	HPV43	L1	GTTGGCTGTACACCTCCTAT	GGG	F	469	488	50	41.63
89	HPV43	L1	ATTCCAGTCCTCTAATAATG	TGG	R	307	326	35	40.39
90	HPV44	L1	ATATACTTTAATACACCCAG	TGG	F	856	875	30	53.66
91	HPV44	L1	TCACTAGTATATGTAGACGG	AGG	R	440	459	40	52.54
92	HPV44	L1	ATGCTGGTATTTTAGAACAG	TGG	F	1175	1194	35	50.57
93	HPV44	L1	GAAGATACAAGAGAACCACT	GGG	R	611	630	40	50.07
94	HPV44	L1	GGGTGGCTTTTGACATGTAA	TGG	R	217	236	45	49.8
95	HPV44	L1	GGCATCCGTAGGTATTACTT	TGG	R	1429	1448	45	49.07
96	HPV44	L1	CCACTAATACCAACACCTAA	GGG	R	1157	1176	40	48.24
97	HPV44	L1	GGGTTGTCGCCGCCCCCAAA	TGG	F	1204	1223	70	45.87
98	HPV44	L1	GTAGGTAGAGGACAGCCCTT	AGG	F	310	329	55	44.86
99	HPV44	L1	ACACTAGTAATTAATTCCAA	GGG	R	932	951	25	43.95
100	HPV44	L1	GGTATGGGCCTGCATCGGGC	TGG	F	285	304	70	43.87
101	HPV44	L1	TTTCCTACCCACACGAACAG	AGG	R	58	77	50	43.79
102	HPV44	L1	ATAATGTTAGTGTTAAGGAT	GGG	F	518	537	25	42.99
103	HPV44	L1	GTAATAAAAATTTTCTACCA	AGG	R	102	121	20	42.63
104	HPV44	L1	GAGTTGGATCAATATCCCCT	TGG	F	1363	1382	45	42.56
105	HPV44	L1	ATATCCTGACTATTTACAAA	TGG	F	681	700	25	42.45
106	HPV44	L1	GGCCCGTTCCTCTGTTCGTG	TGG	F	1416	1435	65	40.86
107	HPV44	L1	GGATGGGGACTGCCCTCCCT	TGG	F	534	553	70	40.46
108	HPV44	L1	GTAGCAGTGCATATATCTAA	TGG	R	830	849	35	40.27
109	HPV54	L1	GGAGTATATTAGACATGTGG	AGG	F	1068	1087	40	62.33
110	HPV54	L1	GGGATTCATTCCATGAATAT	AGG	R	328	347	35	53.99
111	HPV54	L1	GATGTTACCATAGAGCCACT	AGG	R	608	627	45	45.56
112	HPV54	L1	GCAAGCGTGCTAAAACTAAA	AGG	F	1475	1494	40	45.05
113	HPV54	L1	GTTCCAGTCCTCTAGAATAG	TGG	R	307	326	45	44.32
114	HPV54	L1	ATTTATGCTGCAACTCCTAG	TGG	F	856	875	40	43.88
115	HPV54	L1	ACAGGTGTGGAGGTTGGTAG	GGG	F	304	323	55	42.88
116	HPV54	L1	GCTGACAACCTTAGAAACTG	GGG	R	1432	1451	45	41.99
117	HPV54	L1	GTAAATTCATATATACAGGA	TGG	F	565	584	25	41.76
118	HPV54	L1	AGTAAAAACTTGCGACCCAA	GGG	R	104	123	40	41.19
119	HPV54	L1	CCTATGTACACCTAATACAT	TGG	F	513	532	35	40.53
120	HPV61	L1	GGAGGACAGTCAGTGGGCCG	GGG	R	956	975	70	67.27
121	HPV61	L1	GGATGACTGGAACTTTGGTG	TGG	F	1200	1219	50	65.83
122	HPV61	L1	GGGGCATTGAGGTTGGTAGG	GGG	F	314	333	60	64.55
123	HPV61	L1	ATAGTGGTGCAAATATCCAA	TGG	R	833	852	35	53.91
124	HPV61	L1	GGAATAAATATAACTACCAG	GGG	R	640	659	30	51.94
125	HPV61	L1	ATAACTTTAGACACAGGAGT	GGG	R	1454	1473	35	49.74
126	HPV61	L1	GGCTTTATATTCAGATACAG	GGG	R	445	464	35	49.52
127	HPV61	L1	CCACTAGTACCAACCCCCAA	TGG	R	1157	1176	55	48.43
128	HPV61	L1	GGGGGATGTAGCAGTACAAA	TGG	R	466	485	50	48.39
129	HPV61	L1	CGTGACACAGATACTGAGCG	GGG	R	71	90	55	47.49
130	HPV61	L1	GCGATCCTCCTTGGGCGGCG	GGG	R	193	212	75	47.01
131	HPV61	L1	GGAATATTTGCGCCATACAG	AGG	F	1086	1105	45	46.72
132	HPV61	L1	GGAGGATGTAGGGGTAGAGC	TGG	R	34	53	60	46.64
133	HPV61	L1	GTACTGATAACTTTAGACAC	AGG	R	1448	1467	35	46.4
134	HPV61	L1	GGGAATAAATATAACTACCA	GGG	R	639	658	30	45.94
135	HPV61	L1	GATGTCCTACAGTAAGCAGA	CGG	R	1377	1396	45	45.51
136	HPV61	L1	GCCACCATAATAAAATAAGT	TGG	R	1405	1424	30	44.69
137	HPV61	L1	GTATCCTGATTATTTACAAA	TGG	F	693	712	25	43.93
138	HPV61	L1	TGTGTCTTCTAAACTGGTAG	AGG	R	265	284	40	43.69
139	HPV61	L1	GTATTTGTAAATTCCAATGG	AGG	R	938	957	30	43.64
140	HPV61	L1	CAAGAAAAACACTATCCCCA	AGG	F	177	196	40	43.61
141	HPV61	L1	TAAGGGTGCGAATGACAAGG	CGG	F	834	853	50	43.41
142	HPV61	L1	ATATTGATAGCCAGACACCT	TGG	R	1303	1322	40	43.37
143	HPV61	L1	GGTTGGTAGGGGGCAGCCAT	TGG	F	324	343	65	42.46
144	HPV61	L1	TTTATATTCAGATACAGGGG	GGG	R	448	467	35	42.4
145	HPV61	L1	ACAAATACCACCATACAGGA	TGG	F	574	593	40	42.07
146	HPV61	L1	GCTTTATATTCAGATACAGG	GGG	R	446	465	35	41.98
147	HPV61	L1	GGACTCTGCAACAAGTAATG	TGG	R	1096	1115	45	41.66
148	HPV61	L1	AGGAAACTGATCCAAATCAG	TGG	R	124	143	40	41.62
149	HPV61	L1	GGGGAATAAATATAACTACC	AGG	R	638	657	35	41.16
150	HPV61	L1	GCTATGCCAAGTTATCCTTT	TGG	F	1325	1344	40	40.41
151	HPV70	L1	ATAGGTGTGGAAATTGGTAG	AGG	F	307	326	40	61.65
152	HPV70	L1	GGAATATACTAGGCATGTGG	AGG	F	1080	1099	45	53.98
153	HPV70	L1	GGTATGGGCCTGTATAGGTG	TGG	F	294	313	55	53.19
154	HPV70	L1	GTATATTCCCCTTCCCCAAG	TGG	F	862	881	50	48.25
155	HPV70	L1	GCTGACGCAGGGCGTTTGCG	AGG	R	53	72	70	47.07
156	HPV70	L1	ACAACCTTCGCCACAGAAGG	GGG	R	1451	1470	55	45.96
157	HPV70	L1	CCACTAACGCCAACGCCCAA	TGG	R	1160	1179	60	45.23
158	HPV70	L1	TATAAAGGGAAGGATCCGGA	AGG	R	1242	1261	45	42.29
159	HPV70	L1	GGTGTCTGCATATCAGTATA	GGG	F	192	211	40	42.15
160	HPV70	L1	GTGTCAGGATTATAAAGGGA	AGG	R	1232	1251	40	41.6
161	HPV70	L1	TGGACAATCGCCCTGTTGAG	TGG	R	964	983	55	40.95
162	HPV70	L1	GGATCCTTTTTTTCAGGTGT	AGG	R	200	219	40	40.14
163	HPV72	L1	GGATGACTGGAATTTTGGTG	TGG	F	1203	1222	45	65.52
164	HPV72	L1	GGGGTCGAGGTAGAGCTTGG	TGG	R	35	54	65	51.94
165	HPV72	L1	CCGCTAGTGCCAACACCAAG	GGG	R	1163	1182	60	50.11
166	HPV72	L1	ATAGGGTGCAAGCCTCCCAT	TGG	F	475	494	55	48.28
167	HPV72	L1	GTATCATCCAAACTGGTAGA	AGG	R	266	285	40	47.43
168	HPV72	L1	GGTAGCGACAGGGTGACACC	TGG	F	844	863	65	46.5
169	HPV72	L1	TTTACAATCCAGATACTGAA	CGG	F	272	291	30	46.07
170	HPV72	L1	TGTCACCCTGTCGCTACCCG	AGG	R	661	680	65	45.38
171	HPV72	L1	ACACCATTCCTAAGGTTTCG	GGG	F	182	201	45	44.27
172	HPV72	L1	GGTTTCGGGGTATCAATACA	GGG	F	195	214	45	43.91
173	HPV72	L1	ACGAGACTGCAAAAACCTAT	AGG	R	244	263	40	42.1
174	HPV72	L1	GGACACCATAGAGCCGCTGG	GGG	R	613	632	65	41.5
175	HPV72	L1	AGGTGCCTCGGGTAGCGACA	GGG	F	834	853	65	40.91
176	HPV72	L1	GCATATGGATCTTCTTTAGG	AGG	R	188	207	40	40.71
177	HPV72	L1	TGAGAATTAGAGCCTGCACA	AGG	R	980	999	45	40.36
178	HPV72	L1	GCCACCATAATAATAGAGGT	TGG	R	1408	1427	40	40.3
179	HPV81	L1	GCAGTGACAGAATAACCCCC	GGG	F	845	864	55	69.76
180	HPV81	L1	GGCATAAATATAACTCCCGG	GGG	R	634	653	45	65.65
181	HPV81	L1	ATATGTGTCATCTAAACTGG	TGG	R	259	278	35	59.74
182	HPV81	L1	GGGCATAAATATAACTCCCG	GGG	R	633	652	45	59.25
183	HPV81	L1	GATGCCCTACAGTAAGAAGG	CGG	R	1374	1393	50	57.08
184	HPV81	L1	GGCAGTGACAGAATAACCCC	CGG	F	844	863	55	56.99
185	HPV81	L1	GGGGCATAAATATAACTCCC	GGG	R	632	651	45	53.47
186	HPV81	L1	GTACTGATACCCAGACACCT	TGG	R	1303	1322	50	52.53
187	HPV81	L1	ATAACTTTAGACACAGGTGT	GGG	R	1451	1470	35	51.92
188	HPV81	L1	GGAATTTCTGCGCCATACAG	AGG	F	1086	1105	50	51
189	HPV81	L1	CCTGCAGGAAAATAAATCGG	AGG	F	636	655	45	49.07
190	HPV81	L1	GGCCCTGCAGGAAAATAAAT	CGG	F	633	652	45	48.06
191	HPV81	L1	GTATTTTTAAACTCCAAGGG	GGG	R	938	957	35	47.95
192	HPV81	L1	CTACGCCCAAAGGCTGTCCG	CGG	R	1167	1186	65	47.34
193	HPV81	L1	AGGGGCATTGAGGTAGGCCG	CGG	F	310	329	65	46.71
194	HPV81	L1	GGGTTATATAAGGTACCATC	AGG	R	1235	1254	40	46.55
195	HPV81	L1	CCACTAGTACCTACGCCCAA	AGG	R	1157	1176	55	46.54
196	HPV81	L1	GTACTGATAACTTTAGACAC	AGG	R	1445	1464	35	46.4
197	HPV81	L1	CTACTTACATAATATGAACA	AGG	F	1170	1189	25	46.3
198	HPV81	L1	GGCCCAGTGCTCCCCAATGG	GGG	R	1006	1025	70	44.13
199	HPV81	L1	GGTTGTATTTTTAAACTCCA	AGG	R	934	953	30	43.81
200	HPV81	L1	TTGTATTTTTAAACTCCAAG	GGG	R	936	955	25	43.55
201	HPV81	L1	GTATCCAGATTATTTACAAA	TGG	F	690	709	25	43.12
202	HPV81	L1	GGTGGTATTGCAAATATCCA	AGG	R	832	851	40	42.37
203	HPV81	L1	AAAGCGTTCCACTATTCCCA	AGG	F	174	193	45	42.22
204	HPV81	L1	GCCCCCATAATAAAAAAGGT	TGG	R	1402	1421	40	41.21
205	HPV81	L1	GGAGCTTCACACGAAACACC	CGG	R	1281	1300	55	40.98
206	HPV81	L1	GGCAGACGCAGGACGCTTAC	GGG	R	43	62	65	40.77
207	HPV81	L1	GGCAGCAGCACCCTTTTGAC	AGG	R	211	230	60	40.22