HPVomics

A therapeutic and epitome resource for Human Papillomaviruses

Efficient putative sgRNAs (CRISPR/Cas9 targets)

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S.No.	HPV type	Target region	sgRNA seuences	PAM	Strand(F/R)	Start	End	GC (%)	Predicted effeciency (%)
1	HPV6	E4	CCTTGTGTCCTCAAGCACCA	AGG	F	131	150	55	62.24
2	HPV6	E4	GGTGCTTGAGGACACAAGGG	TGG	R	182	201	60	54.31
3	HPV6	E4	CTTGGTGCTTGAGGACACAA	GGG	R	179	198	50	44.35
4	HPV6	E4	GTATGTAGTAGATTCAGGAA	TGG	R	221	240	35	44.24
5	HPV6	E4	GTAGTGCTAGATACAGATGC	AGG	R	255	274	45	43.37
6	HPV6	E4	GCTTGAGGACACAAGGGTGG	AGG	R	185	204	60	41.11
7	HPV11	E4	GGTGCAGGCGGACACTGTAG	GGG	R	176	195	65	62.19
8	HPV11	E4	GTATGTAGTAGGTTCAGCAA	TGG	R	218	237	40	47.95
9	HPV11	E4	TACAGTGTCCGCCTGCACCA	CGG	F	134	153	60	46.98
10	HPV11	E4	TGTGGCCAACATCAGATCCG	TGG	F	221	240	55	40.41
11	HPV40	E4	GGGGGCGTCGGGGGTGTCGG	TGG	R	203	222	85	55.62
12	HPV40	E4	GGTGTCGGTGGCAGTGGTCG	GGG	R	215	234	70	51.97
13	HPV40	E4	GGCGTCAGCAGTCTCAACAA	TGG	R	242	261	55	51.54
14	HPV40	E4	GGCGGGGCCGACCGTTTCGG	TGG	R	170	189	80	49.52
15	HPV40	E4	CCTGACCCTGAAAGCAACAA	CGG	F	285	304	50	47.63
16	HPV40	E4	ACAGCGACCACCGAAACGGT	CGG	F	147	166	60	44.97
17	HPV40	E4	ACTGTGGGCAAATCACTCGG	AGG	F	228	247	55	44.53
18	HPV40	E4	GGGGTGTCGGTGGCAGTGGT	CGG	R	213	232	70	43.71
19	HPV40	E4	GGGCACAGGAACTGTGGTGG	AGG	F	306	325	65	42.93
20	HPV42	E4	GCGTAGCCTGACTTCCACAG	TGG	R	7	26	60	57.86
21	HPV43	E4	GGTGCACGTGGAATCTGGGA	CGG	R	194	213	60	58
22	HPV43	E4	GGTTGTGCCCTCTAGGGTGG	TGG	R	25	44	65	54.21
23	HPV43	E4	CACCCTAGAGGGCACAACCG	TGG	F	303	322	65	50
24	HPV43	E4	AGATTCAGTAGACGCAACAG	AGG	R	236	255	45	43.05
25	HPV43	E4	GCGTCTACTGAATCTGACAC	CGG	F	96	115	50	42.67
26	HPV44	E4	CCGCCATGTAAACGACCCCG	AGG	F	249	268	65	62.31
27	HPV44	E4	GGTGGTGGTGGTGGAGGAGG	AGG	R	227	246	70	61.72
28	HPV44	E4	GGATGAGGCCTGTGCAAGGG	TGG	R	209	228	65	58.85
29	HPV44	E4	ACTGCCCATTGGCACCGCCG	AGG	F	212	231	70	51.37
30	HPV44	E4	TGTCAGAAACAGCGACTCCG	TGG	F	311	330	55	47.81
31	HPV44	E4	GCCTCATCCACACTGCCCAT	TGG	F	201	220	60	47.12
32	HPV44	E4	GGCGTTGTTGGTGTTTGTGG	GGG	R	122	141	55	45.08
33	HPV44	E4	GGCCTGTGCAAGGGTGGTGG	TGG	R	215	234	70	43.56
34	HPV44	E4	TGGCACCGCCGAGGACTGCG	TGG	F	221	240	75	41.36
35	HPV44	E4	AATATGGGAACTCTTTACAA	TGG	F	55	74	30	40.41
36	HPV54	E4	GGAGTTGTCGGTACGTGGTG	TGG	R	176	195	60	66.64
37	HPV54	E4	ACACAGCACCTCAGACCCCG	TGG	F	299	318	65	60.12
38	HPV54	E4	GGACAGTGGGAGGTGCGTAT	GGG	F	87	106	60	49.71
39	HPV54	E4	CTAGCTCGTTTTCTAGGCGG	CGG	R	132	151	55	48.47
40	HPV54	E4	GTATTATGTGGATTTTAAAA	AGG	F	41	60	20	40.44
41	HPV61	E4	GGGTGGACAGTACTGACCAG	TGG	F	229	248	60	70.14
42	HPV61	E4	GGGGGTGGTGGTGGTCTGTG	TGG	R	206	225	70	58.23
43	HPV61	E4	GGGTGGGGCCCACGCACGTG	GGG	R	187	206	80	53.11
44	HPV61	E4	GGTGGGGCCCACGCACGTGG	GGG	R	188	207	80	51.46
45	HPV61	E4	GGGGCCCACGCACGTGGGGG	TGG	R	191	210	85	47.19
46	HPV70	E4	GTAAAGGCTGCAACAAACGA	CGG	F	202	221	45	49.91
47	HPV70	E4	GGTGCATGGGGTTGTTGCGG	CGG	R	140	159	65	41.48
48	HPV70	E4	TGTTGCGGCGGTATGGGCCG	GGG	R	152	171	70	40.12
49	HPV72	E4	GGCGTGACAGTGCTGACCAG	TGG	F	232	251	65	56.48
50	HPV72	E4	GGGGCCCACGCACGTGGCGG	TGG	R	200	219	85	47.26
51	HPV81	E4	GGGCCCCGCCAAGACCACCA	AGG	F	116	135	75	62.19
52	HPV81	E4	GGTGGACAATAACAAATCAG	GGG	F	224	243	40	62.05
53	HPV81	E4	GGTACTGGTGGCGGGTTCGG	GGG	R	203	222	70	53.39
54	HPV81	E4	GGGGCCTTGGTGGTCTTGGC	GGG	R	168	187	70	50.02
55	HPV81	E4	GGGCCTTGGTGGTCTTGGCG	GGG	R	169	188	70	49.45
56	HPV81	E4	GGGGCCCACGTGCTGGGTAC	TGG	R	188	207	75	45.48
57	HPV81	E4	TGCACCACCGAACATCTGGG	TGG	F	206	225	60	44.83
58	HPV81	E4	GGGTGGACAATAACAAATCA	GGG	F	223	242	40	40.85