HPVomics

A therapeutic and epitome resource for Human Papillomaviruses

Efficient putative sgRNAs (CRISPR/Cas9 targets)

---

S.No.	HPV type	Target region	sgRNA seuences	PAM	Strand(F/R)	Start	End	GC (%)	Predicted effeciency (%)
1	HPV6	E7	GGTCTAATACAATATCCTTT	AGG	R	255	274	30	53.83
2	HPV11	E7	GGTCTAGTACTATATCCTTT	AGG	R	255	274	35	57.32
3	HPV11	E7	CTGTGCACTCCACAACCAGT	CGG	R	81	100	55	50.65
4	HPV11	E7	AGAAGATGAGGTGGACAAGG	TGG	F	99	118	50	41.66
5	HPV42	E7	GTGTCCTTTGTGTGCCCGCG	TGG	F	255	274	65	41.53
6	HPV43	E7	ACCGCTGTGGTGGTACACAG	TGG	R	5	24	60	46.32
7	HPV43	E7	CCCACTGTGTACCACCACAG	CGG	F	273	292	60	43.99
8	HPV54	E7	TATAGTCGTGAAGCACAGGT	GGG	R	2	21	45	40.82
9	HPV61	E7	AGACAGCTCAGAGTCAGAGG	AGG	F	87	106	55	40.83
10	HPV70	E7	TGGGTTCCCGATGCACACCA	GGG	R	5	24	60	52.59
11	HPV70	E7	GTTGTTGGTGATTAACTACA	TGG	R	180	199	35	50.29
12	HPV72	E7	CCACATCAGGAAGTTCCTGA	AGG	R	246	265	50	43.95
13	HPV72	E7	GGGTGGTTACTACCTGTGGC	AGG	F	173	192	60	43.22
14	HPV81	E7	GCAATTGCTAGACAGCTCAG	AGG	F	78	97	50	57.09
15	HPV81	E7	GATGTGGATGATAGGGACAG	TGG	F	109	128	50	40.17