Efficient putative sgRNAs (CRISPR/Cas9 targets)

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S.No.	sgRNA seuences	PAM	Start	End	GC (%)	Predicted efficiency (%)
1	GGTGATGCTGTTGTTTACCG	AGG	16852	16871	50	81.37
2	GGTAATCTAGACACACCGGT	TGG	23350	23369	50	78.9
3	GGATTGGCTTCGATGTCGAG	GGG	18296	18315	55	74.36
4	GGATCGAGAGACTTCACCAT	AGG	15409	15428	50	70.57
5	ACTTCTCGTCGTCTTCACCG	TGG	26730	26749	55	69.86
6	GCTGCAACAAAACTAGCGCG	GGG	1509	1528	55	69.15
7	GGTCATGTCATTCTTCCGTA	CGG	22845	22864	45	67.34
8	GGTACTGAAGATGATTACCA	AGG	3113	3132	40	64.49
9	ACAAAGTTGACCAAAACACG	AGG	23576	23595	40	62.27
10	ACTTTGGTCCCTGTTCCGAG	AGG	29614	29633	55	62.25
11	GAGCTCCCTTAAATTCCAGA	AGG	1426	1445	45	61.34
12	GTATTCTGTCAAATTTACAG	AGG	13220	13239	30	60.82
13	TTTCATTGGAAACAACCACG	TGG	27165	27184	40	60.36
14	TATGAGTGTGACATACCCAT	TGG	23540	23559	40	59.82
15	GCTCTTCTCCGAACTGACGG	CGG	1077	1096	60	59.63
16	GTACAGCTGTTTTAAGACAG	TGG	20900	20919	40	59.44
17	CGTGGTCCAGAACAAACCCA	AGG	29102	29121	55	58.97
18	AGATCCATCAAGAATCCTAG	GGG	15936	15955	40	58.93
19	GGGTGATGCGTATTATGACA	TGG	11241	11260	45	58.8
20	GTAGATGTTGGACAACCTAA	AGG	17329	17348	40	58.65
21	GGTGTGACCGAAAGGTAAGA	TGG	247	266	50	58.5
22	GGTCAACTTAGACTCCCAGG	TGG	1557	1576	55	58.08
23	GGACTTTTAAAGTTTCCATT	TGG	27260	27279	30	57.9
24	GGTAAAGGCCAACAACAACA	AGG	28979	28998	45	57.9
25	GGACACTTTGATGGACAACA	GGG	19657	19676	45	57.8
26	GTAAAAGTCGAACATTTCAA	CGG	11855	11874	30	57.77
27	GGCTTAGTAGAAGTTGAAAA	AGG	419	438	35	57.7
28	GAGCCACCACATTTTCACCG	AGG	29715	29734	55	57.27
29	GGTGGTTTACATCTACTGAT	TGG	20356	20375	40	56.99
30	GCAGCCCGTCTTACACCGTG	CGG	13486	13505	65	56.89
31	GGTGGTATCTTAAACGAACA	AGG	14674	14693	40	56.28
32	TTAAAAAGCTCCTTGAACAA	TGG	26560	26579	30	56.18
33	GCTAGCTCTTGGAGGTTCCG	TGG	21138	21157	60	56.03
34	TTCATTGGTGTTCATCACCG	TGG	28560	28579	45	55.93
35	GGAACTGTAACTTTGAAGCA	AGG	25423	25442	40	55.82
36	GCTACCAGACGAATTCGTGG	TGG	28541	28560	55	55.6
37	GGGGAGCTGTAGCTTCGGTT	AGG	11586	11605	60	55.6
38	GTTACAACACTGAAAAACGA	TGG	21511	21530	35	55.35
39	ACATTTTCACCGAGGCCACG	CGG	29723	29742	55	54.65
40	GGGAGAACAGGAGTCTAGAT	CGG	17572	17591	50	54.55
41	ACAAGTGGTTATAAGGTCCG	TGG	28062	28081	45	54.46
42	GGTAGAATGGAAAGCCAGTG	TGG	29635	29654	50	54.45
43	GGTGTTTGTGTATCTACTAG	TGG	9248	9267	40	54.26
44	GGTGGTAGTTATCGGAACAT	AGG	21195	21214	45	54.26
45	CGTCCATTAACTCGTCCCAG	CGG	12448	12467	55	54.11
46	GGTTTAATAACTCATAAAAG	TGG	10058	10077	25	53.34
47	CGTGGTTTAAGGTTTCCAAA	TGG	26748	26767	40	52.87
48	GCTTTCAACCAACCAAACAA	TGG	29859	29878	40	52.78
49	GGAGGTTTCCGTTATCACGC	TGG	28416	28435	55	52.65
50	GGAACAAGCAAATTCTATGG	TGG	15208	15227	40	52.04
51	AGTTTACTGAAATCTAGCCG	CGG	29208	29227	40	51.81
52	TCACTATATGTTAAACCAGG	TGG	15454	15473	35	51.77
53	GTAATGGCCTACATTACAGG	TGG	2054	2073	45	51.74
54	AATTCACCACCAGATCCAAA	TGG	13138	13157	40	51.64
55	CCATTGGTAGATTTGCCAAT	AGG	22235	22254	40	51.54
56	GGGAACTTCTCCTGCTAGAA	TGG	28882	28901	50	51.51
57	AAATTTTGTGAACTTTAACG	TGG	5409	5428	25	51.27
58	GGGACTCAACTTGTAATGGT	CGG	19621	19640	45	51.18
59	AGAATGGTCATGGTCCACCA	GGG	12803	12822	50	50.95
60	GGGTGTATTATTCGACGTCG	TGG	7539	7558	50	50.86
61	ACAGGTGCTTCCTAGTGTCG	AGG	2939	2958	55	50.68
62	GATGATATCGTAAAAACAGA	TGG	15973	15992	30	50.64
63	ACAAAGTCCTCAGAATACAA	AGG	5825	5844	35	50.55
64	ATAGGTCCAGACATGTTCCT	CGG	17530	17549	45	50.47
65	ACACCACTAATTCAACCTAT	TGG	9359	9378	35	49.89
66	ATTGATCGCGTATATGGACG	TGG	6321	6340	45	49.67
67	CTCATGCGTGAGCTTAACGG	AGG	782	801	55	49.63
68	AATTTCGTATCTGCTCCAGA	CGG	16070	16089	40	49.6
69	GGTGTCAGTGTTATAACACC	AGG	23342	23361	45	49.59
70	CTATTGTTAATGGTGTTAGA	AGG	7539	7558	30	49.47
71	AATACCAGTGGCTTACCGCA	AGG	604	623	50	49.36
72	GGTAAGATCGTCCTCTTCAA	GGG	1000	1019	45	49.3
73	ATTGTTATACCATGACCGAA	TGG	20838	20857	35	49.23
74	GCTAACTCTTTGGTGGACAG	AGG	19185	19204	50	49.08
75	AGATGCTTATTACAGAGCAA	GGG	5353	5372	35	48.89
76	TTATGATTGATGTTCAACAA	TGG	18758	18777	25	48.86
77	GGACACATCCAGTTGGTGCA	AGG	627	646	55	48.55
78	CTTTAGTTAAAGGTAAACTG	AGG	9645	9664	30	48.29
79	TTGTTCAGTTGACTTCGCAG	TGG	2079	2098	45	48.27
80	TCATGCGTGAGCTTAACGGA	GGG	783	802	50	47.89
81	TCTGAATTACTTACACCACT	GGG	2924	2943	35	47.83
82	ATAGATCATCCAAATCCTAA	AGG	13265	13284	30	47.06
83	GTAGAAGCTGTTATGTACAT	GGG	5558	5577	35	47.02
84	GGTATAGAATTTCTTAAGAG	AGG	4835	4854	30	46.9
85	AATTACGTGAGTTCTCCCAT	CGG	29034	29053	40	46.62
86	GGAGCTGGTGGCCATAGTTA	CGG	653	672	55	46.59
87	GTTAGTGCTAAACCACCGCC	TGG	18445	18464	55	46.46
88	GTATCGAGAAAAGTCTTGCA	AGG	28891	28910	40	46.46
89	CGAAGAGGTTAATTACACTG	AGG	9772	9791	40	46.41
90	GGGCGCGATCAAAACAACGT	CGG	28374	28393	55	46.37
91	CACACTGGTTTGTAACACAA	AGG	24861	24880	40	46.29
92	GCTCAGTATGAACTTAAGCA	TGG	5699	5718	40	46.23
93	GGTGGTAGTTGTGTTTTAAG	CGG	3563	3582	40	46.22
94	CTATCTGTCTAGCTTAAGAT	GGG	28726	28745	35	46
95	TTATAAGACCAAGATCACAC	GGG	12404	12423	35	45.98
96	TTTGTTACAGACACACCTAA	AGG	12908	12927	35	45.92
97	AGATGTCGTAGACATTACCA	AGG	7473	7492	40	45.72
98	GGACAACAGGTAGTTTCACA	GGG	10226	10245	45	45.67
99	AATACACCAAAAGATCACAT	TGG	28691	28710	30	45.57
100	TTGCTCTTCTGGAACATACG	AGG	27495	27514	45	45.52
101	GCAGTGAAAAGAACAATCAA	GGG	10976	10995	35	45.45
102	GATAGTGCAACATTACCTAA	AGG	20752	20771	35	45.44
103	TCTGGCAATTCACATCAACA	TGG	19761	19780	40	45.39
104	CTAAGGGTAAAAAGTCATAT	TGG	3928	3947	30	45.38
105	GCTAGATTCCCTAAGAGTGA	TGG	12845	12864	45	45.29
106	GGTAACTGTGATGTTGTAAT	AGG	24932	24951	35	45.24
107	GTTGACTTAACCTAAACATA	AGG	8529	8548	30	45.24
108	TGGTGACGATACAAATCACA	AGG	29135	29154	40	45.24
109	ATACATGTCTCTGGGACCAA	TGG	21764	21783	45	45.23
110	GGGCAGACGGTACTTCAAAG	TGG	28638	28657	55	45.22
111	TTTTAGTCCAGAGTACTCAA	TGG	11043	11062	35	44.87
112	GGATTAAACAACCTAAATAG	AGG	12962	12981	30	44.57
113	GGAGAGTACACCTTTGAAAA	AGG	16822	16841	40	44.39
114	AGTAGATGTTTTGTCGGCCG	GGG	13927	13946	50	44.39
115	AAAATTCGTGACAGAAACGG	AGG	25597	25616	40	44.24
116	GTAGTTTATTCATCATACAT	CGG	26911	26930	25	44.22
117	GTATAAACAGGCTAGATCTG	AGG	12301	12320	40	44.21
118	ATTGTAGTAAGGTAATCACT	GGG	18068	18087	30	44.11
119	AACCAGAATGGAGAACGCAG	TGG	28352	28371	50	44.1
120	GGCATGCCTTCTTACTGTAC	TGG	7037	7056	50	44.03
121	ACGTCTTACTAAATACACAA	TGG	13791	13810	30	44.02
122	GTTAATCACTAACCAACAGG	GGG	16758	16777	40	43.99
123	GCAGTCATAACAAGAGAAGT	GGG	8831	8850	40	43.93
124	TTATGCATCTCAAGGTCTAG	TGG	15747	15766	40	43.91
125	TGGAACACTAAACATAGCAG	TGG	746	765	40	43.83
126	TTAACAAGTATTCTTTCACA	CGG	24303	24322	25	43.81
127	TCACAAATATTAGTTATCGG	TGG	23716	23735	30	43.64
128	GAGAAGGTATATCCGTCGAG	AGG	104	123	50	43.62
129	TAGAGTTTCTTAGAGACGGT	TGG	2181	2200	40	43.6
130	GGATACAAGGCTATTGATGG	TGG	8687	8706	45	43.57
131	GGACGATGTGCGACGCTTCG	AGG	2816	2835	65	43.54
132	GTAGAAATTCTACAACTGCA	CGG	29494	29513	35	43.5
133	GGCATTGATACCGGTGGTCG	AGG	29229	29248	60	43.43
134	CTGCACGGAGACTATTCTGG	AGG	29509	29528	55	43.39
135	ATTGGTATCGACTTTAGCCC	CGG	22495	22514	45	43.34
136	GGGATTCTAAAAACTTTAAT	GGG	7771	7790	25	43.32
137	TGAGTCTTAGTTTTCAACAA	TGG	20692	20711	30	43.26
138	GCTATTACCAGAGCAAAAGT	AGG	17926	17945	40	43.2
139	GTAGGACTATTTCTTGTCGT	TGG	6499	6518	40	43.17
140	GATGTGACTCAACTTTACTT	AGG	16411	16430	35	43.08
141	GGAAAAAGTTTCCACATGAG	AGG	13060	13079	40	42.92
142	TCTACACTCCAGGGACCACC	TGG	17068	17087	60	42.88
143	GACCTCGTAGAGAAAACATC	AGG	10004	10023	45	42.88
144	TTTCCACGACTAAAAGATCG	AGG	1841	1860	40	42.83
145	CCAGAAGCTGGACTTCCCTA	TGG	28622	28641	55	42.81
146	TAGTCGTTAGAAAGGTCAAA	CGG	7082	7101	35	42.77
147	TGAATTAGACTCATTCAAGG	AGG	24991	25010	35	42.75
148	ACTGAGACTGACCTTACTAA	AGG	15841	15860	40	42.74
149	GTAGTTCGAAAAAGAAAAGT	TGG	28756	28775	30	42.7
150	CCACTTGGCTATGTAACACA	TGG	4610	4629	45	42.68
151	TCCGATATCGAACATTCCAA	CGG	17803	17822	40	42.66
152	GGAACAACAACAACCGGAAA	TGG	903	922	45	42.56
153	AATTACAATCTAGTCAAGCG	TGG	20651	20670	35	42.48
154	GGACCAAATTGTATATCACT	TGG	14431	14450	35	42.39
155	GGGTGTACTCCCTGTTCCTG	TGG	29304	29323	60	42.3
156	GGTACAGTATTATGCGTAGT	GGG	18641	18660	40	42.26
157	ACAAGATTACCAACATTTAG	TGG	27336	27355	30	42.26
158	AGTACCAACGAAACATCCAA	TGG	11092	11111	40	42.25
159	ATTAGATGAAGAGCAACCAA	TGG	27354	27373	35	42.13
160	CGATGAAACTATGTTCCAAA	CGG	18072	18091	35	42.1
161	GGCTAGATCTGAGGACAAGA	GGG	12310	12329	50	42.06
162	ATCGTGCTACAACTTCCTCA	AGG	28742	28761	45	41.98
163	GCTAAGCACTATGTGTACAT	TGG	17413	17432	40	41.94
164	GTGAATTATCGAAATCCCAA	TGG	10846	10865	35	41.94
165	GGTAGAGATTAACTCCAACT	TGG	26596	26615	40	41.87
166	TCTGAACATGACTACCAGAT	TGG	25930	25949	40	41.78
167	TTACCATAAACATTACGTCG	TGG	5649	5668	35	41.75
168	GGTGTAGAGTTTCTTAGAGA	CGG	2177	2196	40	41.59
169	TTGCGCGTACGCGTTCCATG	TGG	26830	26849	60	41.54
170	CTATTCACTTCTATTCTAAA	TGG	28006	28025	25	41.46
171	CTACGACTATACAGGTTTCG	TGG	20502	20521	45	41.45
172	AGAATAAATTGGATCACCGG	TGG	26736	26755	40	41.38
173	GGTTCACTATATGTTAAACC	AGG	15451	15470	35	41.26
174	CTACCGTGGACACATCCAGT	TGG	620	639	55	41.17
175	ATACACATTACATTAAACTG	AGG	4521	4540	25	41.09
176	GGTGATGCTGGCATGACTTA	CGG	29337	29356	50	41.05
177	CGACCAAATTCATATTACAG	AGG	23400	23419	35	41.02
178	AGAAAACCACATAAGTTCCG	AGG	1200	1219	40	40.92
179	CTATCGTTAAGGTGGCCACT	AGG	3136	3155	50	40.92
180	ACCAGTCTCTGAAGAAGTAG	TGG	6400	6419	45	40.9
181	GTGAGTTTAGTATTAAACAC	CGG	12512	12531	30	40.84
182	CGTCCGTGAAGACAACGTAA	TGG	16854	16873	50	40.73
183	GGCAATTATGTTTGACCACA	TGG	27279	27298	40	40.73
184	GGATAATGTTGTAGTGTCAA	TGG	4950	4969	35	40.72
185	GCCGCTATAACAATACTAGA	TGG	1958	1977	40	40.7
186	CAACTGCACTATATACACCA	TGG	16112	16131	40	40.67
187	ATACACTTCTGCACTGTTAG	CGG	24178	24197	40	40.64
188	TGGACTTTATTGACACTAAG	AGG	921	940	35	40.63
189	GTGTAATCATTGTTTCCGAC	AGG	8655	8674	40	40.59
190	ATAGCTGTATCGCTCACATA	CGG	29077	29096	40	40.56
191	CTAAGAAGTGTAAAACTAAA	TGG	21984	22003	25	40.54
192	TTAGGTTTGGAAAGTGTCAA	AGG	28153	28172	35	40.52
193	ATTCGTTGACTTAAAAGACG	TGG	6222	6241	35	40.48
194	GTTGTTGGAGAAGGTTCCGA	AGG	1577	1596	50	40.47
195	CATTAAATCACACTAAAAAG	TGG	5211	5230	25	40.45
196	CACCACCGAGAAAGTTCAGG	AGG	180	199	55	40.41
197	ACGTGGCTTTGGAGACTCCG	TGG	349	368	60	40.34
198	GGCATTCAGTACGGTCGTAG	TGG	545	564	55	40.33
199	GTCATGTCTGTTGTGCTACG	TGG	16650	16669	50	40.21
200	TTAGATAATCAAGATCTCAA	TGG	14059	14078	25	40.19
201	ATCAGTGTGTAACATTAGGG	AGG	29684	29703	40	40.15
202	GGTGGACCCTCAGATTCAAC	TGG	28325	28344	55	40.12
203	TCTAGAAAGTAAAATGGCAG	TGG	1320	1339	35	40.12

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