RNAi screen analysis of viral host genes
| Gene | vhfRNAi Accession | Vhf12247 |
| Gene Symbol | cnk | |
| Gene Description | Connector enhancer of kinase suppressor of Ras 2 | |
| Gene Name in Paper | CG6556 | |
| Group | Host Factor | |
| Ensembl Gene ID | ENSG00000149970 | |
| UniProt ID | Q8WXI2 | |
| Entrez Gene ID | 22866 | |
| GO | View | |
| Host | Organism | Homo sapiens |
| Virus | Virus full name | West Nile virus |
| Virus Abbreviation | WNV | |
| Taxonomy | View | |
| Results | Phenotype | Increased infection |
| Hits Number* | 537(376 viral sensitivity factors and 161 viral resistance factors; 217 for secondary screen) | |
| Confirmed Hits Number | 145 | |
| Score | 2.25 | |
| Unit | Z-score | |
| Methods | Assay Type | Cell-based |
| BioModel Description | Drosophila DL1 cells for genome-wide RNAi screening in duplicate using dsRNAs against beta-galactosidase (beta gal) as a negative control, and dsRNA against the WNV genome as a positive control. | |
| Confirmatory Screen Description | Confirmed hits were validated by genome-wide RNAi screening in duplicate, and defined as genes for which Robust Z-scores in each duplicate viral infection set of >=1.5 or <=-1.5 in duplicate (~40% change) (p <0.009). | |
| Primary Screen Description# | Any gene for which a robust Z score of >=2 or<=-2 in both replicates (p<0.001;~40% change), was defined as a hit. | |
| Note/Comment | Increased infection(50) | |
| References | Pubmed ID | 24550726 |