RNAi screen analysis of viral host genes
| Gene | vhfRNAi Accession | Vhf12199 |
| Gene Symbol | eIF4G | |
| Gene Description | Eukaryotic translation initiation factor 4 gamma 1 | |
| Gene Name in Paper | CG10811 | |
| Group | Restriction Factor | |
| Ensembl Gene ID | ENSG00000114867 | |
| UniProt ID | Q04637 | |
| Entrez Gene ID | 1981 | |
| GO | View | |
| Host | Organism | Homo sapiens |
| Virus | Virus full name | West Nile virus |
| Virus Abbreviation | WNV | |
| Taxonomy | View | |
| Results | Phenotype | Inhibited infection |
| Hits Number* | 537 | |
| Confirmed Hits Number | 145 | |
| Score | NA | |
| Unit | NA | |
| Methods | Assay Type | Cell-based |
| BioModel Description | Drosophila DL1 cells for genome-wide RNAi screening in duplicate using dsRNAs against beta-galactosidase (beta gal) as a negative control, and dsRNA against the WNV genome as a positive control. | |
| Confirmatory Screen Description | Confirmed hits were validated by genome-wide RNAi screening in duplicate, and defined as genes for which Robust Z-scores in each duplicate viral infection set of >=1.5 or <=-1.5 in duplicate (~40% change) (p <0.009). | |
| Primary Screen Description# | Any gene for which a robust Z score of >=2 or<=-2 in both replicates (p<0.001;~40% change), was defined as a hit.The 537 hits include 376 viral sensitivity factors and 161 viral resistance factors, and 217 were used for secondary screen. | |
| Note/Comment | Inhibited infection(95) | |
| References | Pubmed ID | 24550726 |