RNAi screen analysis of viral host genes
| Gene | vhfRNAi Accession | Vhf11988 |
| Gene Symbol | RFWD2 | |
| Gene Description | E3 ubiquitin-protein ligase RFWD2 | |
| Gene Name in Paper | RFWD2 | |
| Group | Restriction Factor | |
| Ensembl Gene ID | ENSG00000143207 | |
| UniProt ID | Q8NHY2 | |
| Entrez Gene ID | 64326 | |
| GO | View | |
| Host | Organism | Homo sapiens |
| Virus | Virus full name | West Nile virus |
| Virus Abbreviation | WNV | |
| Taxonomy | View | |
| Results | Phenotype | Inhibited infection |
| Hits Number* | Not mentioned | |
| Confirmed Hits Number | 306 | |
| Score | NA | |
| Unit | NA | |
| Methods | Assay Type | Cell-based |
| BioModel Description | HeLa cells for screening a genome-scale pooled siRNA library of 21,121 human genes (a pool of four siRNAs per gene) | |
| Confirmatory Screen Description | Confirmed hits were validated using pooled single siRNA and cytotoxicity assay | |
| Primary Screen Description# | Any gene for which a minimum of two siRNAs reduced (HSF) or increased (HRF) the percentage of infected cells by >2-fold, and the fold change was >2 times the standard deviation (s.d.) of the percentage of control cells infected, was scored as a hit. | |
| Note/Comment | Inhibited infection(283) | |
| References | Pubmed ID | 18690214 |