Vhf11775 details

Gene vhfRNAi AccessionVhf11775
Gene SymbolTOX
Gene DescriptionThymocyte selection-associated high mobility group box protein TOX
Gene Name in PaperTOX
GroupRestriction Factor
Ensembl Gene IDENSG00000198846 
UniProt IDO94900
Entrez Gene ID9760
GOView
Host OrganismHomo sapiens
Virus Virus full nameVesicular stomatitis virus
Virus AbbreviationVSV
TaxonomyView
Results PhenotypeInhibited infection
Hits Number*233 (173 without ribosomal protein genes)
Confirmed Hits Number72
ScoreNA
UnitNA
Methods Assay Type Cell-based
BioModel DescriptionFour independent siRNAs, grouped into a 2 × 2 pool format, targeting each of 22,909 mammalian genes, were used. VSV-eGFP, a recombinant virus encoding enhanced green fluorescent protein (eGFP), was used to infect HeLa cells.
Confirmatory Screen DescriptionConfirmed hits were validated using 72 out of 233 siRNAs from another source, Dharmacon ONTARGETplus pool of four siRNAs per gene.
Primary Screen Description#A hit is defined as a siRNAi whose has Z' factor value of >0.5. Wells with low cell number due to combined effects of siRNA toxicity and VSV cytopathic effects were excluded.
Note/CommentInhibited infection(72)
References Pubmed ID22065774
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*Replicates that are beyond 2-3 standard deviation values from the mean (Sharma 2009)
#A strong signal/background ratio with low variation in the assay (Zhang 1999)