RNAi screen analysis of viral host genes
| Gene | vhfRNAi Accession | Vhf11762 |
| Gene Symbol | PKMYT1 | |
| Gene Description | Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase | |
| Gene Name in Paper | PKMYT1 | |
| Group | Restriction Factor | |
| Ensembl Gene ID | ENSG00000127564 | |
| UniProt ID | Q99640 | |
| Entrez Gene ID | 9088 | |
| GO | View | |
| Host | Organism | Homo sapiens |
| Virus | Virus full name | Vesicular stomatitis virus |
| Virus Abbreviation | VSV | |
| Taxonomy | View | |
| Results | Phenotype | Inhibited infection |
| Hits Number* | 233 (173 without ribosomal protein genes) | |
| Confirmed Hits Number | 72 | |
| Score | NA | |
| Unit | NA | |
| Methods | Assay Type | Cell-based |
| BioModel Description | Four independent siRNAs, grouped into a 2 × 2 pool format, targeting each of 22,909 mammalian genes, were used. VSV-eGFP, a recombinant virus encoding enhanced green fluorescent protein (eGFP), was used to infect HeLa cells. | |
| Confirmatory Screen Description | Confirmed hits were validated using 72 out of 233 siRNAs from another source, Dharmacon ONTARGETplus pool of four siRNAs per gene. | |
| Primary Screen Description# | A hit is defined as a siRNAi whose has Z' factor value of >0.5. Wells with low cell number due to combined effects of siRNA toxicity and VSV cytopathic effects were excluded. | |
| Note/Comment | Inhibited infection(72) | |
| References | Pubmed ID | 22065774 |