RNAi screen analysis of viral host genes
| Gene | vhfRNAi Accession | Vhf11290 |
| Gene Symbol | RASA1 | |
| Gene Description | Ras GTPase-activating protein 1 | |
| Gene Name in Paper | RASA1 | |
| Group | Restriction Factor | |
| Ensembl Gene ID | ENSG00000145715 | |
| UniProt ID | P20936 | |
| Entrez Gene ID | 5921 | |
| GO | View | |
| Host | Organism | Homo sapiens |
| Virus | Virus full name | Vaccinia virus |
| Virus Abbreviation | VACV | |
| Taxonomy | View | |
| Results | Phenotype | Inhibited infection |
| Hits Number* | Not mentioned | |
| Confirmed Hits Number | 576 | |
| Score | 50 | |
| Unit | % Active siRNAs | |
| Methods | Assay Type | Cell-based |
| BioModel Description | HeLa cells for screening siGenome from Dharmacon (DGW), comprising a single pool of four siRNAs for 18,120 genes, and Ambion Silencer Select Version 4 (AGW), a nonpooled library of three independent siRNAs for 21,566 genes. A recombinant VACV IHD-J/GFP d | |
| Confirmatory Screen Description | Confirmed hits were defined as a gene with significant decrease in virus spread with at least two different siRNA reagents (from a third company, Qiagen). | |
| Primary Screen Description# | A <-1.5 MAD decrease in virus spread with at least two different siRNA reagents from the same or different libraries and a similar number with an increase in virus spread were considered hits. The siRNAs inducing a >50% reduction in cell count were filter | |
| Note/Comment | Inhibited infection (576) | |
| References | Pubmed ID | 23401514 |