RNAi screen analysis of viral host genes
| Gene | vhfRNAi Accession | Vhf10959 |
| Gene Symbol | GPR132 | |
| Gene Description | Probable G-protein coupled receptor 132 | |
| Gene Name in Paper | GPR132 | |
| Group | Restriction Factor | |
| Ensembl Gene ID | ENSG00000183484 | |
| UniProt ID | Q9UNW8 | |
| Entrez Gene ID | 29933 | |
| GO | View | |
| Host | Organism | Homo sapiens |
| Virus | Virus full name | Vaccinia virus |
| Virus Abbreviation | VACV | |
| Taxonomy | View | |
| Results | Phenotype | Inhibited infection |
| Hits Number* | 266 | |
| Confirmed Hits Number | 188 | |
| Score | NA | |
| Unit | NA | |
| Methods | Assay Type | Cell-based |
| BioModel Description | HeLa cells for screening the 7,000 druggable genome library (QIAGEN), three nonoverlapping siRNAs against each gene, and repeated independently three times | |
| Confirmatory Screen Description | siRNAs from a different vendor (Ambion) were used to validate confirmed hits using three additional siRNAs in a secondary follow-up screen (repeated independently three times). | |
| Primary Screen Description# | A hit was defined as a gene with two or three of the three siRNAs reduced VACV infection relative to controls (i.e., for the majority, the median over the triplicates of at least two siRNAs had a median absolute deviation less than -1.5 over the whole sc | |
| Note/Comment | Inhibited infection(188) | |
| References | Pubmed ID | 23084750 |