RNAi screen analysis of viral host genes
| Gene | vhfRNAi Accession | Vhf10779 |
| Gene Symbol | DDX58 | |
| Gene Description | Probable ATP-dependent RNA helicase DDX58 | |
| Gene Name in Paper | RIG-I | |
| Group | Host Factor | |
| Ensembl Gene ID | ENSG00000107201 | |
| UniProt ID | O95786 | |
| Entrez Gene ID | 23586 | |
| GO | View | |
| Host | Organism | Homo sapiens |
| Virus | Virus full name | Sendai virus |
| Virus Abbreviation | SeV | |
| Taxonomy | View | |
| Results | Phenotype | Increased infection |
| Hits Number* | 581 | |
| Confirmed Hits Number | 59 | |
| Score | 71.40% | |
| Unit | Target gene inhibition | |
| Methods | Assay Type | Cell-based |
| BioModel Description | Embryonic kidney HEK 293T cells for screening lentiviral-based short hairpin RNA (shRNA) human library of ~15,000 human genes (3 shRNAs per gene). | |
| Confirmatory Screen Description | Confirmed hits were validated using independent siRNA and qRT-PCR. | |
| Primary Screen Description# | Assessing the transcriptional response at the SeV-induced interferon-b (IFNB1) promoter activity | |
| Note/Comment | Increased infection(46) | |
| References | Pubmed ID | 23785285 |