Vhf10568 details

Gene vhfRNAi AccessionVhf10568
Gene SymbolPELI1
Gene DescriptionE3 ubiquitin-protein ligase pellino homolog 1
Gene Name in PaperPELI1
GroupRestriction Factor
Ensembl Gene IDENSG00000197329 
UniProt IDQ96FA3
Entrez Gene ID57162
GOView
Host OrganismHomo sapiens
Virus Virus full nameRotavirus
Virus AbbreviationRV
TaxonomyView
Results PhenotypeInhibited infection
Hits Number*Not mentioned
Confirmed Hits Number522
ScoreNA
UnitNA
Methods Assay Type Cell-based
BioModel DescriptionMonkey kidney MA104 cells (Cercopithecus aethiops epithelial kidney origin) for screening 21,181 genes
Confirmatory Screen DescriptionConfirmed hits were validated using siRNA and qRT-PCR.
Primary Screen Description#An optimized immunofluorescent detection of viral protein synthesis was used to evaluate virus replication.
Note/CommentInhibited infection (522)
References Pubmed ID23733942
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*Replicates that are beyond 2-3 standard deviation values from the mean (Sharma 2009)
#A strong signal/background ratio with low variation in the assay (Zhang 1999)