RNAi screen analysis of viral host genes
| Gene | vhfRNAi Accession | Vhf05463 |
| Gene Symbol | PRSS35 | |
| Gene Description | Inactive serine protease 35 | |
| Gene Name in Paper | PRSS35 | |
| Group | Restriction Factor | |
| Ensembl Gene ID | ENSG00000146250 | |
| UniProt ID | Q8N3Z0 | |
| Entrez Gene ID | 167681 | |
| GO | View | |
| Host | Organism | Homo sapiens |
| Virus | Virus full name | Influenza A virus |
| Virus Abbreviation | IAV | |
| Taxonomy | View | |
| Results | Phenotype | Inhibited infection |
| Hits Number* | 19628-1936(624 after evidence analysis) | |
| Confirmed Hits Number | 298 | |
| Score | NA | |
| Unit | NA | |
| Methods | Assay Type | Cell-based |
| BioModel Description | Human lung epithelial (A549) cells for screening genome-wide libraries comprising 98,737 synthetic siRNAs targeting 19,628 unique human genes were arrayed in 384-well plates (7 ng per siRNA) such that each well contained either two (47,560 wells) or one ( | |
| Confirmatory Screen Description | siRNAs for reconfirmation of 624 genes were individually rearrayed such that each well of a 384-well plate contained a single siRNA (7 ng). Virus replication was monitored by measuring luciferase activities at the indicated times. | |
| Primary Screen Description# | With a hit criterion of an activity score <0.4, 1936 genes were defined as primary hits for the A549 influenza virus screen. | |
| Note/Comment | Inhibited infection_post-entry factors(10) | |
| References | Pubmed ID | 20027183 |