RNAi screen analysis of viral host genes
| Gene | vhfRNAi Accession | Vhf04812 |
| Gene Symbol | SLC35A1 | |
| Gene Description | CMP-sialic acid transporter | |
| Gene Name in Paper | SLC35A1 | |
| Group | Restriction Factor | |
| Ensembl Gene ID | ENSG00000164414 | |
| UniProt ID | P78382 | |
| Entrez Gene ID | 10559 | |
| GO | View | |
| Host | Organism | Homo sapiens |
| Virus | Virus full name | Influenza A virus |
| Virus Abbreviation | IAV | |
| Taxonomy | View | |
| Results | Phenotype | Inhibited infection |
| Hits Number* | 334 | |
| Confirmed Hits Number | 133 | |
| Score | NA | |
| Unit | NA | |
| Methods | Assay Type | Cell-based |
| BioModel Description | Osteosarcoma cells (U2OS) for screening Dharmacon siRNA library targeting 17,877 genes (four siRNAs per gene) in triplicate | |
| Confirmatory Screen Description | Four unique siRNAs from each pool were rescreened separately. 260 out of 334 total pools with at least one siRNA scoring and 133 confirmed with two or more siRNAs (40%), reducing the probability of off-target effects. | |
| Primary Screen Description# | The percentage of HA-positive cells was less than 55% of the plate mean, and cell numbers were not less than 40% of the plate mean | |
| Note/Comment | Inhibited infection(129) | |
| References | Pubmed ID | 20064371 |