RNAi screen analysis of viral host genes
| Gene | vhfRNAi Accession | Vhf04649 |
| Gene Symbol | POLR2L | |
| Gene Description | DNA-directed RNA polymerases I, II, and III subunit RPABC5 | |
| Gene Name in Paper | POLR2L | |
| Group | Restriction Factor | |
| Ensembl Gene ID | ENSG00000177700 | |
| UniProt ID | P62875 | |
| Entrez Gene ID | 5441 | |
| GO | View | |
| Host | Organism | Homo sapiens |
| Virus | Virus full name | Influenza A virus |
| Virus Abbreviation | IAV | |
| Taxonomy | View | |
| Results | Phenotype | Inhibited infection |
| Hits Number* | 287 | |
| Confirmed Hits Number | 168 | |
| Score | NA | |
| Unit | NA | |
| Methods | Assay Type | cell-based |
| BioModel Description | A549 human lung epithelial cells for screening a genome-wide siRNA library consisting of approximately 62,000 siRNAs targeting 17,000 annotated genes and 6,000 predicted genes | |
| Confirmatory Screen Description | Confirmed hits were validated through siRNA and host cell viability determination by WST-1 assay | |
| Primary Screen Description# | Z-scores below -2 | |
| Note/Comment | Inhibited infection_total(168). Primary hits were validated using highly pathogenic avian-origin influenza A virus of the H5N1 subtype (A/Vietnam/1203/2004). | |
| References | Pubmed ID | 20081832 |