Vhf04642 details

Gene vhfRNAi AccessionVhf04642
Gene SymbolPHF2
Gene DescriptionLysine-specific demethylase PHF2
Gene Name in PaperPHF2
GroupRestriction Factor
Ensembl Gene IDENSG00000197724 
UniProt IDO75151
Entrez Gene ID5253
GOView
Host OrganismHomo sapiens
Virus Virus full nameInfluenza A virus
Virus AbbreviationIAV
TaxonomyView
Results PhenotypeInhibited infection
Hits Number*287
Confirmed Hits Number168
ScoreNA
UnitNA
Methods Assay Type cell-based
BioModel DescriptionA549 human lung epithelial cells for screening a genome-wide siRNA library consisting of approximately 62,000 siRNAs targeting 17,000 annotated genes and 6,000 predicted genes
Confirmatory Screen DescriptionConfirmed hits were validated through siRNA and host cell viability determination by WST-1 assay
Primary Screen Description#Z-scores below -2
Note/CommentInhibited infection_total(168). Primary hits were validated using highly pathogenic avian-origin influenza A virus of the H5N1 subtype (A/Vietnam/1203/2004).
References Pubmed ID20081832
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*Replicates that are beyond 2-3 standard deviation values from the mean (Sharma 2009)
#A strong signal/background ratio with low variation in the assay (Zhang 1999)