RNAi screen analysis of viral host genes
| Gene | vhfRNAi Accession | Vhf04475 |
| Gene Symbol | VCP | |
| Gene Description | Transitional endoplasmic reticulum ATPase | |
| Gene Name in Paper | VCP | |
| Group | Restriction Factor | |
| Ensembl Gene ID | ENSG00000165280 | |
| UniProt ID | P55072 | |
| Entrez Gene ID | 7415 | |
| GO | View | |
| Host | Organism | Homo sapiens |
| Virus | Virus full name | Influenza A virus |
| Virus Abbreviation | IAV | |
| Taxonomy | View | |
| Results | Phenotype | Inhibited infection |
| Hits Number* | 178 | |
| Confirmed Hits Number | 77 | |
| Score | -0.6602 | |
| Unit | z-score | |
| Methods | Assay Type | Cell-based |
| BioModel Description | An RNAi library against 13,071 genes (90% of the Drosophila genome) in Drosophila cells. | |
| Confirmatory Screen Description | z-score<=-2.5. siRNA using alternate dsRNAs to control for possible off-target effects. Cell viability was tested using CellTiter-Glo ATP assays. Confirmed hits were validated by a tertiery screen of 110 out of 178 dipteran genes. | |
| Primary Screen Description# | dsRNAs inhibited FVG-R-directed luciferase expression in both replicates | |
| Note/Comment | Inhibited infection(77). Primary hits were validated using WSN virus or H5N1 influenza A/Indonesia/7/05 (Indonesia 7; isolated from a patient). | |
| References | Pubmed ID | 18615016 |