RNAi screen analysis of viral host genes
| Gene | vhfRNAi Accession | Vhf04370 |
| Gene Symbol | TOP3B | |
| Gene Description | DNA topoisomerase 3-beta-1 | |
| Gene Name in Paper | TOP3B | |
| Group | Restriction Factor | |
| Ensembl Gene ID | ENSG00000100038 | |
| UniProt ID | O95985 | |
| Entrez Gene ID | 8940 | |
| GO | View | |
| Host | Organism | Homo sapiens |
| Virus | Virus full name | Human herpesvirus 1 |
| Virus Abbreviation | HSV-1 | |
| Taxonomy | View | |
| Results | Phenotype | Inhibited infection |
| Hits Number* | 358 | |
| Confirmed Hits Number | 72 | |
| Score | NA | |
| Unit | NA | |
| Methods | Assay Type | Cell-based |
| BioModel Description | Epithelial Hela cell line for screening a druggable genome siRNA library (4 siRNAs per gene) targeting 7,237 human genes | |
| Confirmatory Screen Description | Confirmed hits were defined as genes with ≥2 or more siRNAs giving the same or better replication slope than observed in the primary screen, or with <2 of 4 deconvoluted siRNAs reproduced the HSV-1 phenotype observed in the primary screen. | |
| Primary Screen Description# | Hits were defined as the top 2.5% siRNA whose knockdown yielded strong inhibitory phenotypes. | |
| Note/Comment | Inhibited infection(72) | |
| References | Pubmed ID | 23950709 |