RNAi screen analysis of viral host genes
| Gene | vhfRNAi Accession | Vhf04348 |
| Gene Symbol | GBF1 | |
| Gene Description | Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 | |
| Gene Name in Paper | GBF1 | |
| Group | Restriction Factor | |
| Ensembl Gene ID | ENSG00000107862 | |
| UniProt ID | Q92538 | |
| Entrez Gene ID | 8729 | |
| GO | View | |
| Host | Organism | Homo sapiens |
| Virus | Virus full name | Human parainfluenza virus 3 |
| Virus Abbreviation | HPIV-3 | |
| Taxonomy | View | |
| Results | Phenotype | Inhibited infection |
| Hits Number* | Not mentioned | |
| Confirmed Hits Number | 27 | |
| Score | NA | |
| Unit | NA | |
| Methods | Assay Type | Cell-based |
| BioModel Description | Four independent siRNAs, grouped into a 2 × 2 pool format, targeting each of 22,909 mammalian genes, were used. GFPs expressing HPIV was used to infect HeLa cells. | |
| Confirmatory Screen Description | The involvement of the 72 genes identified for VSV was examined in HPIV3 infection. GFPs expressing HPIV3 were used,and the screen was conducted in duplicate. Wells with low cell number due to combined effects of siRNA toxicity and VSV cytopathic effects | |
| Primary Screen Description# | Not proceeded | |
| Note/Comment | Inhibited infection(27) | |
| References | Pubmed ID | 22065774 |