RNAi screen analysis of viral host genes
| Gene | vhfRNAi Accession | Vhf04220 |
| Gene Symbol | CYP3A4 | |
| Gene Description | Cytochrome P450 3A4 | |
| Gene Name in Paper | CYP3A4 | |
| Group | Restriction Factor | |
| Ensembl Gene ID | ENSG00000160868 | |
| UniProt ID | P08684 | |
| Entrez Gene ID | 1576 | |
| GO | View | |
| Host | Organism | Homo sapiens |
| Virus | Virus full name | Human immunodeficiency virus 1 |
| Virus Abbreviation | HIV-1 | |
| Taxonomy | View | |
| Results | Phenotype | Inhibited infection |
| Hits Number* | 390 | |
| Confirmed Hits Number | 232 | |
| Score | NA | |
| Unit | NA | |
| Methods | Assay Type | Cell-based |
| BioModel Description | HeLa P4/R5 cells (which contain ectopically expressed CD4 as well as an integrated HIV LTR-driven b-gal reporter) for screening a siRNA library targeting 19,709 genes with 22,329 pools (3 siRNAs per gene) | |
| Confirmatory Screen Description | The 390 primary screen hits were then screened in silico against a database containing information on expression of over 11,700 genes in multiple tissues, including activated T cells and macrophage. Three hundred and eleven of the primary screen hits were | |
| Primary Screen Description# | A hit was defined as siRNA that increased b-gal activity by at least 2-fold relative to negative controls. | |
| Note/Comment | Inhibited infection(205) | |
| References | Pubmed ID | 18976975 |