RNAi screen analysis of viral host genes
| Gene | vhfRNAi Accession | Vhf04052 |
| Gene Symbol | CD4 | |
| Gene Description | T-cell surface glycoprotein CD4 | |
| Gene Name in Paper | CD4 | |
| Group | Restriction Factor | |
| Ensembl Gene ID | ENSG00000010610 | |
| UniProt ID | P01730 | |
| Entrez Gene ID | 920 | |
| GO | View | |
| Host | Organism | Homo sapiens |
| Virus | Virus full name | Human immunodeficiency virus 1 |
| Virus Abbreviation | HIV-1 | |
| Taxonomy | View | |
| Results | Phenotype | Inhibited infection |
| Hits Number* | Not mentioned | |
| Confirmed Hits Number | 57 | |
| Score | 0.05 | |
| Unit | Density score | |
| Methods | Assay Type | Cell-based |
| BioModel Description | LTR-GFP HeLa CD4+ cell line for screening a whole-genome siRNA library (21000 genes) | |
| Confirmatory Screen Description | Hits were further validated using shRNA-mediated silencing in stably transduced Jurkat cell lines and RT-PCR. Confirmed hits were defined as siRNA that reproduce CD4-like phenotypes upon HIV infection. | |
| Primary Screen Description# | Seven independent genome-wide screens were performed and computed a density score for each siRNA within the CD4 class. For each gene, the ratio between the percentages of experiments inside the CD4 class versus outside was computed. All genes less than a | |
| Note/Comment | Inhibited infection(57) | |
| References | Pubmed ID | 21841144 |