RNAi screen analysis of viral host genes
| Gene | vhfRNAi Accession | Vhf03973 |
| Gene Symbol | RAPGEF1 | |
| Gene Description | Rap guanine nucleotide exchange factor 1 | |
| Gene Name in Paper | RAPGEF1 | |
| Group | Restriction Factor | |
| Ensembl Gene ID | ENSG00000107263 | |
| UniProt ID | Q13905 | |
| Entrez Gene ID | 2889 | |
| GO | View | |
| Host | Organism | Homo sapiens |
| Virus | Virus full name | Human immunodeficiency virus 1 |
| Virus Abbreviation | HIV-1 | |
| Taxonomy | View | |
| Results | Phenotype | Inhibited infection |
| Hits Number* | 336 | |
| Confirmed Hits Number | 273 | |
| Score | 39% | |
| Unit | RLU | |
| Methods | Assay Type | Cell-based |
| BioModel Description | HeLa-derived TZM-bl cells,which express endogenous CXCR4, transgenic CD4, and CCR5, and an integrated Tat-dependent b-galactosidase (b-Gal) reporter genesiRNA, for screening a genome-wide siRNA library containing 21,121 pools (four siRNAs per gene) | |
| Confirmatory Screen Description | Beta-gal siRNAs (rescreening the four siRNAs from each pool separately) were used to validate the hits and confirmed hits were defined as at least one siRNA scoring in either primary or secondary screen | |
| Primary Screen Description# | Pools were classified as hits if they decreased the percentage of p24-positive cells or b-Gal activity by ?2 SDs from the plate mean. And the siRNAs did not decrease viable cells by >2 SDs. | |
| Note/Comment | Inhibited infection(273) | |
| References | Pubmed ID | 18187620 |