RNAi screen analysis of viral host genes
| Gene | vhfRNAi Accession | Vhf03626 |
| Gene Symbol | DUSP16 | |
| Gene Description | Dual specificity protein phosphatase 16 | |
| Gene Name in Paper | DUSP16 | |
| Group | Restriction Factor | |
| Ensembl Gene ID | ENST00000228862 | |
| UniProt ID | Q9BY84 | |
| Entrez Gene ID | 80824 | |
| GO | View | |
| Host | Organism | Homo sapiens |
| Virus | Virus full name | Human immunodeficiency virus 1 |
| Virus Abbreviation | HIV-1 | |
| Taxonomy | View | |
| Results | Phenotype | Inhibited infection |
| Hits Number* | >800 | |
| Confirmed Hits Number | 295 | |
| Score | NA | |
| Unit | NA | |
| Methods | Assay Type | Cell-based |
| BioModel Description | Genome-wide siRNA libraries targeting 19,628 human genes (2 siRNAs/well, 3 wells/gene) were screened in duplicate or triplicate runs by using a high-throughput robotic system to identify siRNAs that influence the infection of human 293T cells by either a | |
| Confirmatory Screen Description | siRNAs for reconfirmation were individually rearrayed in 384 wells in duplicate and were screened in at least 4 independent experiments. And those inhibit HIV (VSV-G) infection by more than 45% with at least 2 siRNAs while not significantly affecting cell | |
| Primary Screen Description# | Genome-wide RNAi screens for genes required for infection by utilizing a single-cycle HIV-1 reporter virus engineered to encode luciferase and bearing the Vesicular Stomatitis Virus Glycoprotein (VSV-G) on its surface to facilitate efficient infection | |
| Note/Comment | Inhibited infection(295) | |
| References | Pubmed ID | 18854154 |