RNAi screen analysis of viral host genes
| Gene | vhfRNAi Accession | Vhf01932 |
| Gene Symbol | WWTR1 | |
| Gene Description | WW domain-containing transcription regulator protein 1 | |
| Gene Name in Paper | WWTR1 | |
| Group | Restriction Factor | |
| Ensembl Gene ID | ENSG00000018408 | |
| UniProt ID | Q9GZV5 | |
| Entrez Gene ID | 25937 | |
| GO | View | |
| Host | Organism | Homo sapiens |
| Virus | Virus full name | Hepatitis C virus |
| Virus Abbreviation | HCV | |
| Taxonomy | View | |
| Results | Phenotype | Inhibited infection |
| Hits Number* | 521 | |
| Confirmed Hits Number | 262 | |
| Score | NA | |
| Unit | NA | |
| Methods | Assay Type | Cell-based |
| BioModel Description | A wholegenome siRNA library (Dharmacon siGenome, 19,470 pools of four siRNAs per gene) screen in Huh 7.5.1 human hepatocellular cell line | |
| Confirmatory Screen Description | Four siRNAs from each pool were tested separately using quantitative RT-PCR and imaging assay and found that 262 out of the 521 total pools selected (50%) were confirmed with at least two siRNAs. | |
| Primary Screen Description# | Pools were classified as hits (decreased infection) if the percentage of infected cells was <50% of the plate mean in either part one and two, or in part two alone, and did not decrease cell numbers to 50% of the plate mean in part one. | |
| Note/Comment | Inhibited infection(237) | |
| References | Pubmed ID | 19717417 |