RNAi screen analysis of viral host genes
| Gene | vhfRNAi Accession | Vhf01717 |
| Gene Symbol | CDK8 | |
| Gene Description | Cyclin-dependent kinase 8 | |
| Gene Name in Paper | CDK8 | |
| Group | Restriction Factor | |
| Ensembl Gene ID | ENSG00000132964 | |
| UniProt ID | P49336 | |
| Entrez Gene ID | 1024 | |
| GO | View | |
| Host | Organism | Homo sapiens |
| Virus | Virus full name | Hepatitis C virus |
| Virus Abbreviation | HCV | |
| Taxonomy | View | |
| Results | Phenotype | Inhibited infection |
| Hits Number* | 106 | |
| Confirmed Hits Number | 58 | |
| Score | -6.361507288 | |
| Unit | Fold change | |
| Methods | Assay Type | Cell-based |
| BioModel Description | A siRNA-based screen silencing 691 human kinases and associated proteins in Huh7 hepatoma cells | |
| Confirmatory Screen Description | To validate the relevance of the identified kinases in the complete infectious viral life cycle, identified hits were confirmed in a secondary screen using cell-culture-derived HCV (HCVcc). False positive results due to toxicity were excluded using MTT-ba | |
| Primary Screen Description# | A primary screen using HCV pseudoparticles (HCVpp) bearing HCV envelope glycoproteins and vesicular stomatitis virus pseudotyped particles (VSVpp) as an unrelated control virus. | |
| Note/Comment | Inhibited entry and infection (58) | |
| References | Pubmed ID | 21516087 |