Vhf01702 details

Gene vhfRNAi AccessionVhf01702
Gene SymbolPDIK1L
Gene DescriptionSerine/threonine-protein kinase PDIK1L
Gene Name in PaperPDIK1L
GroupRestriction Factor
Ensembl Gene IDENSG00000175087 
UniProt IDQ8N165
Entrez Gene ID149420
GOView
Host OrganismHomo sapiens
Virus Virus full nameHepatitis C virus
Virus AbbreviationHCV
TaxonomyView
Results PhenotypeInhibited infection
Hits Number*106
Confirmed Hits Number58
Score-3.16109274
UnitFold change
Methods Assay Type Cell-based
BioModel DescriptionA siRNA-based screen silencing 691 human kinases and associated proteins in Huh7 hepatoma cells
Confirmatory Screen DescriptionTo validate the relevance of the identified kinases in the complete infectious viral life cycle, identified hits were confirmed in a secondary screen using cell-culture-derived HCV (HCVcc). False positive results due to toxicity were excluded using MTT-ba
Primary Screen Description#A primary screen using HCV pseudoparticles (HCVpp) bearing HCV envelope glycoproteins and vesicular stomatitis virus pseudotyped particles (VSVpp) as an unrelated control virus.
Note/CommentInhibited entry and infection (58)
References Pubmed ID21516087
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*Replicates that are beyond 2-3 standard deviation values from the mean (Sharma 2009)
#A strong signal/background ratio with low variation in the assay (Zhang 1999)