RNAi screen analysis of viral host genes
| Gene | vhfRNAi Accession | Vhf01581 |
| Gene Symbol | MAP2K2 | |
| Gene Description | Dual specificity mitogen-activated protein kinase kinase 2 | |
| Gene Name in Paper | MAP2K2 | |
| Group | Restriction Factor | |
| Ensembl Gene ID | ENSG00000126934 | |
| UniProt ID | P36507 | |
| Entrez Gene ID | 5605 | |
| GO | View | |
| Host | Organism | Homo sapiens |
| Virus | Virus full name | Hepatitis C virus |
| Virus Abbreviation | HCV | |
| Taxonomy | View | |
| Results | Phenotype | Inhibited infection |
| Hits Number* | 178 | |
| Confirmed Hits Number | 28 | |
| Score | -0.6364 | |
| Unit | Z-score | |
| Methods | Assay Type | Cell-based |
| BioModel Description | Huh7.5 cells for screening 2157 siRNAs targeting 719 human kinase genes plus positive controls targeting the entry receptor CD81 or the viral genome itself (HCV321 and HCV138) and four different negative controls (non-silencing siRNA) | |
| Confirmatory Screen Description | Wells showing the lowest 5% and highest 5% of firefly reporter activity (correlated to the number of viable cells) were excluded | |
| Primary Screen Description# | Wells with less than 125 and more than 500 cells were excluded | |
| Note/Comment | Inhibited infection(28) | |
| References | Pubmed ID | 20823335 |