RNAi screen analysis of viral host genes
| Gene | vhfRNAi Accession | Vhf01553 |
| Gene Symbol | PRKD1 | |
| Gene Description | Serine/threonine-protein kinase D1 | |
| Gene Name in Paper | PRKD1 | |
| Group | Restriction Factor | |
| Ensembl Gene ID | ENSG00000184304 | |
| UniProt ID | Q15139 | |
| Entrez Gene ID | 5587 | |
| GO | View | |
| Host | Organism | Homo sapiens |
| Virus | Virus full name | Hepatitis C virus |
| Virus Abbreviation | HCV | |
| Taxonomy | View | |
| Results | Phenotype | Inhibited infection |
| Hits Number* | Not mentioned | |
| Confirmed Hits Number | 14 | |
| Score | 73 | |
| Unit | % inhibition | |
| Methods | Assay Type | Cell-based |
| BioModel Description | Huh-7.5 cells for screening a library consisted of 122 siRNAs targeted to 140 membrane trafficking genes and 18 additional genes important for replication and membrane organization in other viral systems | |
| Confirmatory Screen Description | To limit the possibility of siRNA off-target effects, individual siRNAs were tested. Cell viability using a luminescence-based assay was quantified that measures cellular ATP levels, following 5 days of silencing to eliminate phenotypes resulting from sig | |
| Primary Screen Description# | In fluorescence microscopy assay, the siRNAs that inhibited extracellular infectious HCV production by more than 1 standard deviation of siRNA treated cells (>65% inhibition) were classified as hits and were characterized in secondary assays. | |
| Note/Comment | Inhibited infection(14) | |
| References | Pubmed ID | 22241992 |