Vhf00398 details

Gene vhfRNAi AccessionVhf00398
Gene SymbolSPT25
Gene DescriptionSpermatogenesis-associated protein 25 (Testis-specific gene 23 protein)
Gene Name in PaperC20ORF165
GroupRestriction Factor
Ensembl Gene IDENST00000372519  
UniProt IDQ9BR10
Entrez Gene ID128497
GOView
Host OrganismHomo sapiens
Virus Virus full nameAdeno-associated virus
Virus AbbreviationAAV 
TaxonomyView
Results PhenotypeInhibited transduction
Hits Number*1483
Confirmed Hits Number968 
ScoreNA
UnitNA
Methods Assay Type cell-based
BioModel DescriptionHEK293 and HeLa cells
Confirmatory Screen DescriptionImmunofluorescence
Primary Screen Description#log 2 Luc/AB ratio >2
Note/CommentHost restriction factors critical for in vivo AAV transduction
References Pubmed ID26305933
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*Replicates that are beyond 2-3 standard deviation values from the mean (Sharma 2009)
#A strong signal/background ratio with low variation in the assay (Zhang 1999)