Pipeline-1:: sgRNA-Scanner-- geCRISPRc -- geCRISPR-analysis
Pipeline-2:: sgRNA-Scanner-- geCRISPRr -- geCRISPR-analysis
Following are the salient features and components of ge-CRISPR Web server:
- sgRNA-Scanner = Identification of potential sgRNAs in a gene or genome sequence.
Example sgRNA sequence pattern:
< Distal | Proximal | PAM >
<5'-NNNNNNNN|NNNNNNNNNNNN| NGG- 3'>
Pipeline-1:geCRISPRc = Prediction of sgRNAs as highly or least potent using a new classification based algorithm.
Pipeline-2:geCRISPRr = Prediction of actual sgRNAs efficiency (0-100%) employing a new regression based algorithm.
geCRISPR-analysis = Search and analysis of potential off-targets and structure of the respective sgRNA.