Primer ID (Forward/Reverse) Sequence Start-stop Tm (C)
Frag1.F1.1
Frag1.R1.1		 CACTACATGTACTTGATCCCAGG
TAGAACTCCARGGCCGACATCTG		 7256-7278
7706-7728		 59.89
64.59
Frag1.F1.1
Frag1.R2.1		 CACTACATGTACTTGATCCCAGG
AGTAGAACTCCARGGCCGACATC		 7256-7278
7708-7730		 59.89
64.59
Frag1.F1.1
Frag1.R3.1		 CACTACATGTACTTGATCCCAGG
GTAGAACTCCARGGCCGACATC		 7256-7278
7708-7729		 59.89
63.59
Frag1.F4.1
Frag1.R4.1		 ACTACATGTACTTGATCCCAGG
GAGTAGAACTCCARGGCCGAC		 7257-7278
7711-7731		 58.45
63.09
Frag1.F5.1
Frag1.R5.1		 CACTACATGTACTTGATCCCAG
TAGAACTCCARGGCCGACATC		 7256-7277
7708-7728		 57.84
62.25
Frag2.F1.1
Frag2.R1.1		 TACTGGAACTCCTCYACAGCCAC
CTATGTTCCACCCATAGCTTTGCAC		 7541-7563
8000-8024		 64.29
64.01
Frag2.F1.1
Frag2.R2.1		 TACTGGAACTCCTCYACAGCCAC
GTTCCACCCATAGCTTTGCAC		 7541-7563
8000-8020		 64.29
61.8
Frag2.F1.1
Frag2.R3.1		 TACTGGAACTCCTCYACAGCCAC
TGTTCCACCCATAGCTTTGC		 7541-7563
8002-8021		 64.29
59.93
Frag2.F1.1
Frag2.R4.1		 TACTGGAACTCCTCYACAGCCAC
CTATGTTCCACCCATAGCTTTGC		 7541-7563
8002-8024		 64.29
61.24
Frag2.F1.1
Frag2.R5.1		 TACTGGAACTCCTCYACAGCCAC
TGTTCCACCCATAGCTTTGCACC		 7541-7563
7999-8021		 64.29
64.91
Frag3.F1.1
Frag3.R1.1		 TGGTGCAAAGCTATGGGTGG
CACATCCTCCTCATATTTCACTG		 7998-8017
8403-8425		 62.18
58.56
Frag3.F2.1
Frag3.R2.1		 TGGTGCAAAGCTATGGGTGGAAC
CATCCTCCTCATATTTCACTGGCC		 7998-8020
8400-8423		 65.31
62.7
Frag3.F3.1
Frag3.R3.1		 GGTGCAAAGCTATGGGTGGAAC
TCCTCCTCATATTTCACTGGCCTCC		 7999-8020
8397-8421		 64.11
65.2
Frag3.F2.1
Frag3.R4.1		 TGGTGCAAAGCTATGGGTGGAAC
TCCTCATATTTCACTGGCCTCC		 7998-8020
8397-8418		 65.31
61.1
Frag3.F1.1
Frag3.R5.1		 TGGTGCAAAGCTATGGGTGG
CCTCCTCATATTTCACTGGCCTCC		 7998-8017
8397-8420		 62.18
64.32
Frag4.F1.1
Frag4.R1.1		 CCAGTGAAATATGAGGAGGATG
CTTTTTCCTCTTCAAATATTGC		 8402-8423
8888-8909		 57.97
53.39
Frag4.F2.1
Frag4.R1.1		 GCCAGTGAAATATGAGGAGG
CTTTTTCCTCTTCAAATATTGC		 8401-8420
8888-8909		 57.31
53.39
Frag4.F2.1
Frag4.R3.1		 GCCAGTGAAATATGAGGAGG
TCTTTTTCCTCTTCAAATATTGC		 8401-8420
8888-8910		 57.31
54.78
Frag4.F4.1
Frag4.R1.1		 GGCCAGTGAAATATGAGGAG
CTTTTTCCTCTTCAAATATTGC		 8400-8419
8888-8909		 57.65
53.39
Frag4.F4.1
Frag4.R3.1		 GGCCAGTGAAATATGAGGAG
TCTTTTTCCTCTTCAAATATTGC		 8400-8419
8888-8910		 57.65
54.78
Frag5.F1.1
Frag5.R1.1		 GGRGCAATATTTGAAGAGGAAAAAG
CCACTTTGTTTTGGTATGTG		 8885-8909
9370-9389		 59.65
54.72
Frag5.F1.1
Frag5.R2.1		 GGRGCAATATTTGAAGAGGAAAAAG
ACCACTTTGTTTTGGTATGTG		 8885-8909
9370-9390		 59.65
56.49
Frag5.F3.1
Frag5.R2.1		 GRGCAATATTTGAAGAGGAAAAAG
ACCACTTTGTTTTGGTATGTG		 8886-8909
9370-9390		 57.44
56.49
Frag5.F3.1
Frag5.R1.1		 GRGCAATATTTGAAGAGGAAAAAG
CCACTTTGTTTTGGTATGTG		 8886-8909
9370-9389		 57.44
54.72
Frag5.F5.1
Frag5.R2.1		 RGCAATATTTGAAGAGGAAAAAG
ACCACTTTGTTTTGGTATGTG		 8887-8909
9370-9390		 55.62
56.49
Frag6.F1.1
Frag6.R1.1		 ACACATACCAAAACAAAGTG
CAATCAGTTCATCTTGGTGG		 9369-9388
9847-9866		 53.74
55.86
Frag6.F2.1
Frag6.R2.1		 ACACATACCAAAACAAAGTGG
CAAGAACCTGAGGGCATGTG		 9369-9389
9684-9703		 56.18
60.95
Frag6.F3.1
Frag6.R3.1		 TACACATACCAAAACAAAGTGG
GCCAATCAGTTCATCTTGGTG		 9368-9389
9848-9868		 56.12
59.4
Frag6.F2.1
Frag6.R4.1		 ACACATACCAAAACAAAGTGG
AGGGCATGTGCAAACCTATC		 9369-9389
9674-9693		 56.18
60.12
Frag6.F2.1
Frag6.R3.1		 ACACATACCAAAACAAAGTGG
GCCAATCAGTTCATCTTGGTG		 9369-9389
9848-9868		 56.18
59.4
Frag7.F1.1
Frag7.R1.1		 GTTTGCACATGCCCTCAGGTTCTTG
CAGTCTCCCGGATGCTCCATCC		 9679-9703
9893-9914		 66.83
66.28
Frag7.F2.1
Frag7.R2.1		 ATGATAGGTTTGCACATGCCC
CAATCAGTTCATCTTGGTGGC		 9672-9692
9846-9866		 60.42
59.06
Frag7.F3.1
Frag7.R3.1		 ATAGGTTTGCACATGCCCTC
GCCAATCAGTTCATCTTGGTGGC		 9675-9694
9846-9868		 60.12
64.11
Frag7.F4.1
Frag7.R4.1		 GATGATAGGTTTGCACATGCCCTC
GGTCGTTCTCCTCAATCCAC		 9671-9694
10108-10127		 63.97
59.99
Frag7.F5.1
Frag7.R4.1		 TGATAGGTTTGCACATGCCCTCAG
GGTCGTTCTCCTCAATCCAC		 9673-9696
10108-10127		 64.93
59.99
Frag8.F1.1
Frag8.R1.1		 CCACCAAGATGAACTGATTG
TAGTCCATGTACTTTTCTTC		 9847-9866
10289-10308		 56.17
51.38
Frag8.F2.1
Frag8.R2.1		 CGCCACCAAGATGAACTGATTGG
TCGTTCTCCTCAATCCACAC		 9845-9867
10106-10125		 63.78
58.97
Frag8.F3.1
Frag8.R3.1		 CCACCAAGATGAACTGATTGG
GGTCGTTCTCCTCAATCCACAC		 9847-9867
10106-10127		 58.51
62.86
Frag8.F1.1
Frag8.R4.1		 CCACCAAGATGAACTGATTG
CGTTCTCCTCAATCCACACTC		 9847-9866
10104-10124		 56.17
60.27
Frag8.F3.1
Frag8.R2.1		 CCACCAAGATGAACTGATTGG
TCGTTCTCCTCAATCCACAC		 9847-9867
10106-10125		 58.51
58.97
Frag9.F1.1
Frag9.R1.1		 TGGAGCATCCGGGAGACTGC
GGTCGTTCTCCTCAATCCACAC		 9896-9915
10106-10127		 65.66
62.86
Frag9.F2.1
Frag9.R2.1		 AGTGTGGATTGAGGAGAACG
TCCATGTACTTTTCTTCATCACC		 10105-10124
10283-10305		 58.64
57.83
Frag9.F3.1
Frag9.R3.1		 GTGGATTGAGGAGAACGACC
GTCCATGTACTTTTCTTCATCAC		 10108-10127
10284-10306		 59.62
57.24
Frag9.F2.1
Frag9.R4.1		 AGTGTGGATTGAGGAGAACG
CTTTTCTTCATCACCTATGATCC		 10105-10124
10275-10297		 58.64
56.33
Frag9.F2.1
Frag9.R5.1		 AGTGTGGATTGAGGAGAACG
TTTTCTTCATCACCTATGATCC		 10105-10124
10275-10296		 58.64
54.97
Frag10.F1.1
Frag10.R1.1		 AGAGTGTGGATTGAGGAGAACG
CCATGTACTTTTCTTCATCACC		 10103-10124
10283-10304		 61.1
56.56
Frag10.F1.1
Frag10.R2.1		 AGAGTGTGGATTGAGGAGAACG
TAGTCCATGTACTTTTCTTCATCAC		 10103-10124
10284-10308		 61.1
58.31
Frag10.F3.1
Frag10.R3.1		 AGTGTGGATTGAGGAGAACG
TAGTCCATGTACTTTTCTTCATC		 10105-10124
10286-10308		 58.64
55.39
Frag10.F1.1
Frag10.R4.1		 AGAGTGTGGATTGAGGAGAACG
CTTTTCTTCATCACCTATGATCC		 10103-10124
10275-10297		 61.1
56.33
Frag10.F3.1
Frag10.R5.1		 AGTGTGGATTGAGGAGAACG
GTCCATGTACTTTTCTTCATC		 10105-10124
10286-10306		 58.64
53.95
Frag12.F1.1
Frag12.R1.1		 AGGTGATGAAGAAAAGTACATGGAC
GCTGACTAGCAGGCCTGACAAC		 10282-10306
10381-10402		 60.62
65.45
Frag12.F2.1
Frag12.R2.1		 AGGATCATAGGTGATGAAGAAAAG
CTGACTAGCAGGCCTGACAAC		 10274-10297
10381-10401		 57.87
62.71
Frag12.F1.1
Frag12.R2.1		 AGGTGATGAAGAAAAGTACATGGAC
CTGACTAGCAGGCCTGACAAC		 10282-10306
10381-10401		 60.62
62.71
Frag12.F4.1
Frag12.R2.1		 AGGTGATGAAGAAAAGTACATGG
CTGACTAGCAGGCCTGACAAC		 10282-10304
10381-10401		 57.84
62.71
Frag12.F5.1
Frag12.R2.1		 GGTGATGAAGAAAAGTACATGGAC
CTGACTAGCAGGCCTGACAAC		 10283-10306
10381-10401		 59.54
62.71