Primer ID (Forward/Reverse) Sequence Start-stop Tm (C)
Frag1.F1.1
Frag1.R1.1		 GCCAGAATYGCATGTGTCCTCATTG
ATCATTAGCAGCGGGACTCC		 6758-6782
7175-7194		 65.65
61.08
Frag1.F2.1
Frag1.R2.1		 CCAGAATYGCATGTGTCCTCATTG
CCTGGGATCAAGTACATGTAGTG		 6759-6782
7256-7278		 63.26
60.22
Frag1.F2.1
Frag1.R1.1		 CCAGAATYGCATGTGTCCTCATTG
ATCATTAGCAGCGGGACTCC		 6759-6782
7175-7194		 63.26
61.08
Frag1.F4.1
Frag1.R2.1		 CAGAATYGCATGTGTCCTCATTG
CCTGGGATCAAGTACATGTAGTG		 6760-6782
7256-7278		 61.13
60.22
Frag1.F4.1
Frag1.R1.1		 CAGAATYGCATGTGTCCTCATTG
ATCATTAGCAGCGGGACTCC		 6760-6782
7175-7194		 61.13
61.08
Frag2.F1.1
Frag2.R1.1		 TTGGAGTCCCGCTGCTAATG
TCWATTGTCATTGTGTCAATGTCAG		 7173-7192
7365-7389		 61.94
59.48
Frag2.F1.1
Frag2.R2.1		 TTGGAGTCCCGCTGCTAATG
GGGGTCWATTGTCATTGTGTCAATG		 7173-7192
7369-7393		 61.94
62.41
Frag2.F1.1
Frag2.R3.1		 TTGGAGTCCCGCTGCTAATG
GGTCWATTGTCATTGTGTCAATGTC		 7173-7192
7367-7391		 61.94
60.52
Frag2.F1.1
Frag2.R4.1		 TTGGAGTCCCGCTGCTAATG
GGGTCWATTGTCATTGTGTCAATG		 7173-7192
7369-7392		 61.94
60.32
Frag2.F1.1
Frag2.R5.1		 TTGGAGTCCCGCTGCTAATG
CWATTGTCATTGTGTCAATGTCAG		 7173-7192
7365-7388		 61.94
58.39
Frag3.F1.1
Frag3.R1.1		 CACTACATGTACTTGATCCCAGG
TAGAACTCCARGGCCGACATCTG		 7256-7278
7706-7728		 59.89
64.59
Frag3.F1.1
Frag3.R2.1		 CACTACATGTACTTGATCCCAGG
AGTAGAACTCCARGGCCGACATC		 7256-7278
7708-7730		 59.89
64.59
Frag3.F1.1
Frag3.R3.1		 CACTACATGTACTTGATCCCAGG
GTAGAACTCCARGGCCGACATC		 7256-7278
7708-7729		 59.89
63.59
Frag3.F4.1
Frag3.R4.1		 CACTACATGTACTTGATCCCAG
TAGAACTCCARGGCCGACATC		 7256-7277
7708-7728		 57.84
62.25
Frag3.F5.1
Frag3.R4.1		 ACTACATGTACTTGATCCCAGG
TAGAACTCCARGGCCGACATC		 7257-7278
7708-7728		 58.45
62.25