Primer ID (Forward/Reverse) Sequence Start-stop Tm (C)
Frag1.F1.1
Frag1.R1.1		 ATGGCCTCATAGCCTCGCTCTATC
CTCTCTGTCATGTGTCCTGGC		 6102-6125
6490-6510		 65.5
62.07
Frag1.F2.1
Frag1.R2.1		 GCCTCATAGCCTCGCTCTATCG
CTCTCTGTCATGTGTCCTGG		 6105-6126
6491-6510		 63.88
59.03
Frag1.F3.1
Frag1.R1.1		 CCTCATAGCCTCGCTCTATC
CTCTCTGTCATGTGTCCTGGC		 6106-6125
6490-6510		 58.76
62.07
Frag1.F2.1
Frag1.R1.1		 GCCTCATAGCCTCGCTCTATCG
CTCTCTGTCATGTGTCCTGGC		 6105-6126
6490-6510		 63.88
62.07
Frag1.F5.1
Frag1.R2.1		 CCTCATAGCCTCGCTCTATCG
CTCTCTGTCATGTGTCCTGG		 6106-6126
6491-6510		 61.12
59.03
Frag2.F1.1
Frag2.R1.1		 GAAGATGGTGCTTTGATGGC
TCTCTGTCATGTGTCCTGGC		 6276-6295
6490-6509		 59.03
60.87
Frag2.F2.1
Frag2.R1.1		 AGAAGATGGTGCTTTGATGG
TCTCTGTCATGTGTCCTGGC		 6275-6294
6490-6509		 57.18
60.87
Frag2.F3.1
Frag2.R3.1		 AGAAGATGGTGCTTTGATGGCAC
CTCTCTGTCATGTGTCCTGG		 6275-6297
6491-6510		 63.36
59.03
Frag2.F1.1
Frag2.R4.1		 GAAGATGGTGCTTTGATGGC
CTCTCTGTCATGTGTCCTGGC		 6276-6295
6490-6510		 59.03
62.07
Frag2.F5.1
Frag2.R3.1		 AGAAGATGGTGCTTTGATGGC
CTCTCTGTCATGTGTCCTGG		 6275-6295
6491-6510		 60.31
59.03
Frag3.F1.1
Frag3.R1.1		 CCAACAACACCATAMTGGAAGACAG
TCTCTGTCATGTGTCCTGGC		 6300-6324
6490-6509		 62.87
60.87
Frag3.F1.1
Frag3.R2.1		 CCAACAACACCATAMTGGAAGACAG
CTCTCTGTCATGTGTCCTGGC		 6300-6324
6490-6510		 62.87
62.07
Frag3.F1.1
Frag3.R3.1		 CCAACAACACCATAMTGGAAGACAG
CTCTCTGTCATGTGTCCTGG		 6300-6324
6491-6510		 62.87
59.03
Frag3.F4.1
Frag3.R3.1		 ACCAACAACACCATAMTGGAAGAC
CTCTCTGTCATGTGTCCTGG		 6299-6322
6491-6510		 61.88
59.03
Frag3.F5.1
Frag3.R2.1		 CAACAACACCATAMTGGAAGACAG
CTCTCTGTCATGTGTCCTGGC		 6301-6324
6490-6510		 60.79
62.07
Frag4.F1.1
Frag4.R1.1		 TGCCAGGACACATGACAGAGAG
ATGAGGACACATGCRATTCTGGCTG		 6489-6510
6756-6780		 63.79
66.22
Frag4.F2.1
Frag4.R2.1		 TGCCAGGACACATGACAGAG
ACAATGAGGACACATGCRATTCTGG		 6489-6508
6759-6783		 61.55
64.05
Frag4.F1.1
Frag4.R2.1		 TGCCAGGACACATGACAGAGAG
ACAATGAGGACACATGCRATTCTGG		 6489-6510
6759-6783		 63.79
64.05
Frag4.F2.1
Frag4.R1.1		 TGCCAGGACACATGACAGAG
ATGAGGACACATGCRATTCTGGCTG		 6489-6508
6756-6780		 61.55
66.22
Frag4.F1.1
Frag4.R5.1		 TGCCAGGACACATGACAGAGAG
CAATGAGGACACATGCRATTCTGGC		 6489-6510
6758-6782		 63.79
65.29
Frag5.F1.1
Frag5.R1.1		 GCAGAGACTGGAAGYAGGCC
ACAATGAGGACACATGCRATTCTGG		 6551-6570
6759-6783		 63.29
64.05
Frag5.F1.1
Frag5.R2.1		 GCAGAGACTGGAAGYAGGCC
ATGAGGACACATGCRATTCTGGCTG		 6551-6570
6756-6780		 63.29
66.22
Frag5.F1.1
Frag5.R3.1		 GCAGAGACTGGAAGYAGGCC
TGAGGACACATGCRATTCTGGCTGG		 6551-6570
6755-6779		 63.29
67.57
Frag5.F1.1
Frag5.R4.1		 GCAGAGACTGGAAGYAGGCC
CAATGAGGACACATGCRATTCTGGC		 6551-6570
6758-6782		 63.29
65.29
Frag5.F1.1
Frag5.R5.1		 GCAGAGACTGGAAGYAGGCC
TGAGGACACATGCRATTCTGGCTG		 6551-6570
6756-6779		 63.29
66.09
Frag6.F1.1
Frag6.R1.1		 GCCAGAATYGCATGTGTCCTCATTG
TGGCATCCCTTTGCCCATRCCAAAC		 6758-6782
7132-7156		 65.65
69.03
Frag6.F2.1
Frag6.R2.1		 CCAGCCAGAATYGCATGTGTCCTC
ACTCCAGCTTGYGTGGCCATCGCC		 6755-6778
7105-7128		 66.98
71.94
Frag6.F2.1
Frag6.R3.1		 CCAGCCAGAATYGCATGTGTCCTC
CACTCCAGCTTGYGTGGCCATCGC		 6755-6778
7106-7129		 66.98
71.05
Frag6.F2.1
Frag6.R1.1		 CCAGCCAGAATYGCATGTGTCCTC
TGGCATCCCTTTGCCCATRCCAAAC		 6755-6778
7132-7156		 66.98
69.03
Frag6.F1.1
Frag6.R5.1		 GCCAGAATYGCATGTGTCCTCATTG
CACTCCAGCTTGYGTGGCCATCG		 6758-6782
7107-7129		 65.65
68.88