Primer ID (Forward/Reverse) Sequence Start-stop Tm (C)
Frag2.F1.1
Frag2.R1.1		 ATCTGTGGCATGAACCCAATAGC
CCCCATTGCCATARAGTCCTATCAC		 4526-4548
5036-5060		 62.64
64.05
Frag2.F2.1
Frag2.R1.1		 TGTGGCATGAACCCAATAGC
CCCCATTGCCATARAGTCCTATCAC		 4529-4548
5036-5060		 60.01
64.05
Frag2.F3.1
Frag2.R1.1		 CATCTGTGGCATGAACCCAATAGC
CCCCATTGCCATARAGTCCTATCAC		 4525-4548
5036-5060		 63.84
64.05
Frag2.F4.1
Frag2.R1.1		 CCATCTGTGGCATGAACCCAATAGC
CCCCATTGCCATARAGTCCTATCAC		 4524-4548
5036-5060		 65.78
64.05
Frag2.F5.1
Frag2.R1.1		 CATCTGTGGCATGAACCCAATAG
CCCCATTGCCATARAGTCCTATCAC		 4524-4547
5036-5060		 61.61
64.05
Frag3.F1.1
Frag3.R1.1		 AGACTTGATCCATAYTGGGG
TCACGGACTATTTCAGGAAGAAC		 4790-4809
5210-5232		 56.75
59.74
Frag3.F1.1
Frag3.R2.1		 AGACTTGATCCATAYTGGGG
ACGGACTATTTCAGGAAGAAC		 4790-4809
5210-5230		 56.75
56.98
Frag3.F1.1
Frag3.R3.1		 AGACTTGATCCATAYTGGGG
GCTTCACGGACTATTTCAGGAAG		 4790-4809
5213-5235		 56.75
61.14
Frag3.F1.1
Frag3.R4.1		 AGACTTGATCCATAYTGGGG
GGCTTCACGGACTATTTCAG		 4790-4809
5217-5236		 56.75
58.1
Frag3.F1.1
Frag3.R5.1		 AGACTTGATCCATAYTGGGG
TGGCTTCACGGACTATTTCAGG		 4790-4809
5216-5237		 56.75
61.7
Frag4.F1.1
Frag4.R1.1		 CAGGAGRGTTCTTCCTGAAATAGTC
TCAAAGCCTGAGCTCCAGGC		 5203-5227
5630-5649		 61.51
64.02
Frag4.F2.1
Frag4.R2.1		 GGAGRGTTCTTCCTGAAATAGTCCG
CTGAGCTCCAGGCTCTCTCTGG		 5205-5229
5621-5642		 63.2
65.63
Frag4.F3.1
Frag4.R3.1		 AGGAGRGTTCTTCCTGAAATAGTCC
TGAGCTCCAGGCTCTCTCTG		 5204-5228
5622-5641		 62.03
62.67
Frag4.F3.1
Frag4.R4.1		 AGGAGRGTTCTTCCTGAAATAGTCC
TCAAAGCCTGAGCTCCAGGCTC		 5204-5228
5628-5649		 62.03
66.45
Frag4.F5.1
Frag4.R5.1		 CGATGCTGARGAAGAAGCAGCTAAC
AAAGCCTGAGCTCCAGGCTCTCTC		 5145-5169
5624-5647		 64.81
67.92
Frag5.F1.1
Frag5.R1.1		 CCAGAGAGAGCCTGGAGCTCAGG
ATTCTTGCTTCAAGCCAGTGTGC		 5621-5643
6053-6075		 67.69
63.63
Frag5.F2.1
Frag5.R2.1		 GAGAGCCTGGAGCTCAGGCTTTG
TGCTTCAAGCCAGTGTGCATG		 5626-5648
6050-6070		 67.34
63.7
Frag5.F3.1
Frag5.R3.1		 CCAGAGAGAGCCTGGAGCTCAG
CTTGCTTCAAGCCAGTGTGCATGG		 5621-5641
6049-6072		 66.06
66.64
Frag5.F4.1
Frag5.R4.1		 GAGAGAGCCTGGAGCTCAGG
CGATAGAGCGAGGCTATGAG		 5624-5643
6107-6126		 63.29
58.99
Frag5.F5.1
Frag5.R5.1		 CAGAGAGAGCCTGGAGCTCAGG
TTCTTGCTTCAAGCCAGTGTG		 5622-5643
6054-6074		 65.63
60.97
Frag6.F1.1
Frag6.R1.1		 CCATGCACACTGGCTTGAAGC
GTGCCATCAAAGCACCATCTTC		 6049-6069
6276-6297		 64.28
62.31
Frag6.F1.1
Frag6.R2.1		 CCATGCACACTGGCTTGAAGC
TCTCTGTCATGTGTCCTGGC		 6049-6069
6490-6509		 64.28
60.87
Frag6.F3.1
Frag6.R1.1		 TGCACACTGGCTTGAAGCAAGAATG
GTGCCATCAAAGCACCATCTTC		 6052-6076
6276-6297		 66.18
62.31
Frag6.F4.1
Frag6.R4.1		 ATGCACACTGGCTTGAAGCAAG
TGCCATCAAAGCACCATCTTC		 6051-6072
6276-6296		 63.87
60.92
Frag6.F5.1
Frag6.R5.1		 CCATGCACACTGGCTTGAAG
CTCTCTGTCATGTGTCCTGGC		 6049-6068
6490-6510		 61.79
62.07
Frag7.F1.1
Frag7.R1.1		 GCCTCATAGCCTCGCTCTATC
TCTCTGTCATGTGTCCTGGC		 6105-6125
6490-6509		 61.84
60.87
Frag7.F2.1
Frag7.R2.1		 GATGGCCTCATAGCCTCGCTCTATC
GTGCCATCAAAGCACCATCTTC		 6101-6125
6276-6297		 66.32
62.31
Frag7.F3.1
Frag7.R3.1		 TGGCCTCATAGCCTCGCTCTATC
TGCCATCAAAGCACCATCTTC		 6103-6125
6276-6296		 65.34
60.92
Frag7.F4.1
Frag7.R4.1		 ATGGCCTCATAGCCTCGCTCTATC
CTCTCTGTCATGTGTCCTGGC		 6102-6125
6490-6510		 65.5
62.07
Frag7.F5.1
Frag7.R5.1		 CTCATAGCCTCGCTCTATCG
CTCTCTGTCATGTGTCCTGG		 6107-6126
6491-6510		 58.64
59.03