Gene Primer ID (Forward/Reverse) Sequence Start-stop Tm (C)
1 NS2B
63.82		 Frag1.F1.1
Frag1.R1.1		 TTTGCTTTGGCCTGGTTGGC-3926
GCCATGGGYCCAGCCATCTC-4289		 3945
4308
2 NS2B
56.65		 Frag1.F2.1
Frag1.R2.1		 AAAGGYAGTGTGAAGAAGAAC-4094
AGTATGATCTCTCTCATGGG-4487		 4114
4506
3 NS2B
56.65		 Frag1.F2.1
Frag1.R3.1		 AAAGGYAGTGTGAAGAAGAAC-4094
TGAGTATGATCTCTCTCATGGG-4487		 4114
4508
4 NS2B
56.65		 Frag1.F2.1
Frag1.R4.1		 AAAGGYAGTGTGAAGAAGAAC-4094
TTGAGTATGATCTCTCTCATGG-4488		 4114
4509
5 NS2B
56.65		 Frag1.F2.1
Frag1.R5.1		 AAAGGYAGTGTGAAGAAGAAC-4094
TGAGTATGATCTCTCTCATGG-4488		 4114
4508
6 NS2B
61.05		 Frag2.F1.1
Frag2.R1.1		 ACAGCYGTTGGCCTGATATG-4235
ATTGGGTTCATGCCACAGATG-4525		 4254
4545
7 NS2B
61.05		 Frag2.F1.1
Frag2.R2.1		 ACAGCYGTTGGCCTGATATG-4235
TGGGTTCATGCCACAGATGG-4524		 4254
4543
8 NS2B
61.05		 Frag2.F1.1
Frag2.R3.1		 ACAGCYGTTGGCCTGATATG-4235
TGAGTATGATCTCTCTCATGGG-4487		 4254
4508
9 NS2B
61.05		 Frag2.F1.1
Frag2.R4.1		 ACAGCYGTTGGCCTGATATG-4235
TTGGGTTCATGCCACAGATGG-4524		 4254
4544
10 NS2B
61.05		 Frag2.F1.1
Frag2.R5.1		 ACAGCYGTTGGCCTGATATG-4235
CTATTGGGTTCATGCCACAGATG-4525		 4254
4547
11 NS2B
56.71		 Frag3.F1.1
Frag3.R1.1		 GGAAAGAGTGTGGAYATGTAC-4346
CTATTGGGTTCATGCCACAGATG-4525		 4366
4547
12 NS2B
56.71		 Frag3.F1.1
Frag3.R2.1		 GGAAAGAGTGTGGAYATGTAC-4346
GCTATTGGGTTCATGCCACAG-4528		 4366
4548
13 NS2B
56.71		 Frag3.F1.1
Frag3.R3.1		 GGAAAGAGTGTGGAYATGTAC-4346
TATTGGGTTCATGCCACAGATG-4525		 4366
4546
14 NS2B
56.71		 Frag3.F1.1
Frag3.R4.1		 GGAAAGAGTGTGGAYATGTAC-4346
TTGGGTTCATGCCACAGATG-4525		 4366
4544
15 NS2B
56.71		 Frag3.F1.1
Frag3.R5.1		 GGAAAGAGTGTGGAYATGTAC-4346
CTATTGGGTTCATGCCACAG-4528		 4366
4547
16 NS2B
63.76		 Frag4.F1.1
Frag4.R1.1		 CCATCTGTGGCATGAACCCAATAG-4524
CTCTGTACACTCCATCTGTRGTCTC-4649		 4547
4673
17 NS2B
65.78		 Frag4.F2.1
Frag4.R1.1		 CCATCTGTGGCATGAACCCAATAGC-4524
CTCTGTACACTCCATCTGTRGTCTC-4649		 4548
4673
18 NS2B
63.76		 Frag4.F1.1
Frag4.R3.1		 CCATCTGTGGCATGAACCCAATAG-4524
ACTCTGTACACTCCATCTGTRGTC-4651		 4547
4674
19 NS2B
63.76		 Frag4.F1.1
Frag4.R4.1		 CCATCTGTGGCATGAACCCAATAG-4524
TCTGTACACTCCATCTGTRGTCTC-4649		 4547
4672
20 NS2B
65.78		 Frag4.F2.1
Frag4.R4.1		 CCATCTGTGGCATGAACCCAATAGC-4524
TCTGTACACTCCATCTGTRGTCTC-4649		 4548
4672