Primer ID (Forward/Reverse)	Sequence	Start-stop	Tm (C)
Frag1.F1.1 Frag1.R1.1	TTTGCTTTGGCCTGGTTGGC GCCATGGGYCCAGCCATCTC	3926-3945 4289-4308	63.82 66.89
Frag1.F2.1 Frag1.R2.1	AAAGGYAGTGTGAAGAAGAAC AGTATGATCTCTCTCATGGG	4094-4114 4487-4506	56.65 54.59
Frag1.F2.1 Frag1.R3.1	AAAGGYAGTGTGAAGAAGAAC TGAGTATGATCTCTCTCATGGG	4094-4114 4487-4508	56.65 57.71
Frag1.F2.1 Frag1.R4.1	AAAGGYAGTGTGAAGAAGAAC TTGAGTATGATCTCTCTCATGG	4094-4114 4488-4509	56.65 55.92
Frag1.F2.1 Frag1.R5.1	AAAGGYAGTGTGAAGAAGAAC TGAGTATGATCTCTCTCATGG	4094-4114 4488-4508	56.65 55.13
Frag2.F1.1 Frag2.R1.1	ACAGCYGTTGGCCTGATATG ATTGGGTTCATGCCACAGATG	4235-4254 4525-4545	61.05 60.73
Frag2.F1.1 Frag2.R2.1	ACAGCYGTTGGCCTGATATG TGGGTTCATGCCACAGATGG	4235-4254 4524-4543	61.05 61.92
Frag2.F1.1 Frag2.R3.1	ACAGCYGTTGGCCTGATATG TGAGTATGATCTCTCTCATGGG	4235-4254 4487-4508	61.05 57.71
Frag2.F1.1 Frag2.R4.1	ACAGCYGTTGGCCTGATATG TTGGGTTCATGCCACAGATGG	4235-4254 4524-4544	61.05 62.42
Frag2.F1.1 Frag2.R5.1	ACAGCYGTTGGCCTGATATG CTATTGGGTTCATGCCACAGATG	4235-4254 4525-4547	61.05 61.61
Frag3.F1.1 Frag3.R1.1	GGAAAGAGTGTGGAYATGTAC CTATTGGGTTCATGCCACAGATG	4346-4366 4525-4547	56.71 61.61
Frag3.F1.1 Frag3.R2.1	GGAAAGAGTGTGGAYATGTAC GCTATTGGGTTCATGCCACAG	4346-4366 4528-4548	56.71 61.63
Frag3.F1.1 Frag3.R3.1	GGAAAGAGTGTGGAYATGTAC TATTGGGTTCATGCCACAGATG	4346-4366 4525-4546	56.71 60.47
Frag3.F1.1 Frag3.R4.1	GGAAAGAGTGTGGAYATGTAC TTGGGTTCATGCCACAGATG	4346-4366 4525-4544	56.71 60.33
Frag3.F1.1 Frag3.R5.1	GGAAAGAGTGTGGAYATGTAC CTATTGGGTTCATGCCACAG	4346-4366 4528-4547	56.71 58.51
Frag4.F1.1 Frag4.R1.1	CCATCTGTGGCATGAACCCAATAG CTCTGTACACTCCATCTGTRGTCTC	4524-4547 4649-4673	63.76 62.73
Frag4.F2.1 Frag4.R1.1	CCATCTGTGGCATGAACCCAATAGC CTCTGTACACTCCATCTGTRGTCTC	4524-4548 4649-4673	65.78 62.73
Frag4.F1.1 Frag4.R3.1	CCATCTGTGGCATGAACCCAATAG ACTCTGTACACTCCATCTGTRGTC	4524-4547 4651-4674	63.76 61.97
Frag4.F1.1 Frag4.R4.1	CCATCTGTGGCATGAACCCAATAG TCTGTACACTCCATCTGTRGTCTC	4524-4547 4649-4672	63.76 61.73
Frag4.F2.1 Frag4.R4.1	CCATCTGTGGCATGAACCCAATAGC TCTGTACACTCCATCTGTRGTCTC	4524-4548 4649-4672	65.78 61.73