Primer ID (Forward/Reverse) Sequence Start-stop Tm (C)
Frag1.F1.1
Frag1.R1.1		 TTGGAGGAATGTCCTGGTTCTCAC
GGGGAGTCAGGATGGTACTTGTAC		 2334-2357
2569-2592		 64.13
64.05
Frag1.F2.1
Frag1.R2.1		 TTTGGAGGAATGTCCTGGTTCTCAC
GGGGAGTCAGGATGGTACTTGTACC		 2333-2357
2568-2592		 64.47
65.66
Frag1.F3.1
Frag1.R3.1		 TGGAGGAATGTCCTGGTTCTC
AGTCAGGATGGTACTTGTACC		 2335-2355
2568-2588		 61.06
57.97
Frag1.F4.1
Frag1.R4.1		 AGGAATGTCCTGGTTCTCAC
GGGAGTCAGGATGGTACTTGTACC		 2338-2357
2568-2591		 58.77
63.68
Frag1.F1.1
Frag1.R5.1		 TTGGAGGAATGTCCTGGTTCTCAC
GGAGTCAGGATGGTACTTGTAC		 2334-2357
2569-2590		 64.13
59.57
Frag2.F1.1
Frag2.R1.1		 GGTACAAGTACCATCCTGAC
CTCTCCACATRGGGTTTTTTACAG		 2568-2587
2751-2774		 56.89
60.37
Frag2.F2.1
Frag2.R1.1		 GGTACAAGTACCATCCTGACTC
CTCTCCACATRGGGTTTTTTACAG		 2568-2589
2751-2774		 59.57
60.37
Frag2.F3.1
Frag2.R1.1		 GGTACAAGTACCATCCTGACTCCC
CTCTCCACATRGGGTTTTTTACAG		 2568-2591
2751-2774		 63.68
60.37
Frag2.F4.1
Frag2.R1.1		 GGTACAAGTACCATCCTGACTCC
CTCTCCACATRGGGTTTTTTACAG		 2568-2590
2751-2774		 61.54
60.37
Frag2.F1.1
Frag2.R5.1		 GGTACAAGTACCATCCTGAC
TCTCCACATRGGGTTTTTTACAG		 2568-2587
2751-1773		 56.89
59.22
Frag3.F1.1
Frag3.R1.1		 AGAATGGAAAACATCATGTGG
TCAGCCTCCATGTGTCRTTC		 2660-2680
3094-3113		 55.8
60.6
Frag3.F2.1
Frag3.R2.1		 GAATGGAAAACATCATGTGG
CAYTCCTTCAGTGTGTCACC		 2661-2680
2885-2904		 54.3
58.86
Frag3.F1.1
Frag3.R2.1		 AGAATGGAAAACATCATGTGG
CAYTCCTTCAGTGTGTCACC		 2660-2680
2885-2904		 55.8
58.86
Frag3.F1.1
Frag3.R4.1		 AGAATGGAAAACATCATGTGG
TTGGCCAYTCACATGTTTTCATC		 2660-2680
3133-3155		 55.8
60.8
Frag3.F1.1
Frag3.R5.1		 AGAATGGAAAACATCATGTGG
GCCAYTCACATGTTTTCATCTC		 2660-2680
3131-3152		 55.8
58.84
Frag4.F1.1
Frag4.R1.1		 AAGAAYGACACATGGAGGCTG
GTTGATCTCAGAGATGGTCC		 3092-3112
3359-3378		 61.19
56.63
Frag4.F1.1
Frag4.R2.1		 AAGAAYGACACATGGAGGCTG
TGGGCCTTATCTCCATTCCATAC		 3092-3112
3466-3488		 61.19
61.58
Frag4.F1.1
Frag4.R3.1		 AAGAAYGACACATGGAGGCTG
GGCCTTATCTCCATTCCATAC		 3092-3112
3466-3486		 61.19
57.65
Frag4.F1.1
Frag4.R4.1		 AAGAAYGACACATGGAGGCTG
TGGGCCTTATCTCCATTCCATACC		 3092-3112
3465-3488		 61.19
63.39
Frag4.F1.1
Frag4.R5.1		 AAGAAYGACACATGGAGGCTG
GGGCCTTATCTCCATTCCATAC		 3092-3112
3466-3487		 61.19
60.18
Frag5.F1.1
Frag5.R1.1		 AGGACCATCTCTGAGATCAAC
ACCAGCACTGCCATTGATGTG		 3358-3378
1637-3657		 58.37
63.28
Frag5.F2.1
Frag5.R2.1		 AGAGGACCATCTCTGAGATC
TCTCCTCCAGTGTTCATTTC		 3356-3375
3731-3750		 57.08
56.48
Frag5.F3.1
Frag5.R3.1		 GAGGACCATCTCTGAGATCAAC
TACCAGCACTGCCATTGATG		 3357-3378
3639-3658		 59.63
60.05
Frag5.F2.1
Frag5.R4.1		 AGAGGACCATCTCTGAGATC
CATCTCCTCCAGTGTTCATTTC		 3356-3375
3731-3752		 57.08
58.64
Frag5.F5.1
Frag5.R5.1		 AGAGGACCATCTCTGAGATCAAC
CCAGCACTGCCATTGATGTG		 3356-3378
3637-3656		 60.81
61.87