Primer ID (Forward/Reverse) Sequence Start-stop Tm (C)
Frag1.F1.1
Frag1.R1.1		 ACTTGGGTTGTGTACGGAAC
AAGGTAGGCTTCACCTTGTGTTG		 692-711
1200-1222		 59.77
62.73
Frag1.F2.1
Frag1.R2.1		 ACTTGGGTTGTGTACGGAACC
CTTGTCAAGGTAGGCTTCACC		 692-712
1208-1228		 61.88
60.23
Frag1.F2.1
Frag1.R1.1		 ACTTGGGTTGTGTACGGAACC
AAGGTAGGCTTCACCTTGTGTTG		 692-712
1200-1222		 61.88
62.73
Frag1.F4.1
Frag1.R4.1		 CTTGGGTTGTGTACGGAACC
TTGTCAAGGTAGGCTTCACCTTGTG		 693-712
1203-1227		 60.41
64.82
Frag1.F1.1
Frag1.R5.1		 ACTTGGGTTGTGTACGGAAC
GTCAAGGTAGGCTTCACCTTGTG		 692-711
1203-1225		 59.77
63.31
Frag2.F1.1
Frag2.R1.1		 ACGAGCCAAAAAGTYATATACTTGG
TTGCAGACATATTGAGTGTCTG		 920-944
1233-1254		 59.81
57.98
Frag2.F1.1
Frag2.R2.1		 ACGAGCCAAAAAGTYATATACTTGG
CTCCCTTTGCCAAAAAGTCCAC		 920-944
1290-1311		 59.81
62.24
Frag2.F3.1
Frag2.R3.1		 CCAAAAAGTYATATACTTGGTCATG
GTAGGCTTCACCTTGTGTTGG		 925-949
1199-1219		 57.1
60.75
Frag2.F3.1
Frag2.R4.1		 CCAAAAAGTYATATACTTGGTCATG
GGTAGGCTTCACCTTGTGTTG		 925-949
1200-1220		 57.1
61.13
Frag2.F5.1
Frag2.R5.1		 GAGCCAAAAAGTYATATACTTGGTC
TGCTTGTCAAGGTAGGCTTC		 922-946
1211-1230		 58.88
59.61
Frag3.F1.1
Frag3.R1.1		 CAACACAAGGTGAAGCCTACCTTG
CTTTGTTGTTCCARTGTGGAGTTCC		 1200-1223
1658-1682		 63.93
63.14
Frag3.F2.1
Frag3.R1.1		 ACAAGGTGAAGCCTACCTTGAC
CTTTGTTGTTCCARTGTGGAGTTCC		 1204-1225
1658-1682		 62.02
63.14
Frag3.F3.1
Frag3.R1.1		 GGTGAAGCCTACCTTGACAAGC
CTTTGTTGTTCCARTGTGGAGTTCC		 1208-1229
1658-1682		 63.08
63.14
Frag3.F4.1
Frag3.R1.1		 AAGGTGAAGCCTACCTTGACAAGC
CTTTGTTGTTCCARTGTGGAGTTCC		 1206-1229
1658-1682		 64.44
63.14
Frag3.F5.1
Frag3.R5.1		 AACACAAGGTGAAGCCTACC
TCTTTGTTGTTCCARTGTGGAGTTC		 1201-1220
1659-1683		 59.23
62.41
Frag4.F1.1
Frag4.R1.1		 TGGACTTTTTGGCAAAGGGAG
TCTTTGTTGTTCCARTGTGGAGTTC		 1291-1311
1659-1683		 60.83
62.41
Frag4.F2.1
Frag4.R2.1		 TGTGGACTTTTTGGCAAAGGGAG
CTTTGTTGTTCCARTGTGGAGTTCC		 1289-1231
1658-1682		 63.53
63.14
Frag4.F3.1
Frag4.R1.1		 TGTGGACTTTTTGGCAAAGG
TCTTTGTTGTTCCARTGTGGAGTTC		 1289-1308
1659-1683		 58.46
62.41
Frag4.F4.1
Frag4.R2.1		 GTGGACTTTTTGGCAAAGGG
CTTTGTTGTTCCARTGTGGAGTTCC		 1290-1309
1658-1682		 59.46
63.14
Frag4.F2.1
Frag4.R1.1		 TGTGGACTTTTTGGCAAAGGGAG
TCTTTGTTGTTCCARTGTGGAGTTC		 1289-1231
1659-1683		 63.53
62.41
Frag5.F1.1
Frag5.R1.1		 GGAACTCCACAYTGGAACAACAAAG
TGCATGTCCACCGCCATCTG		 1658-1682
1994-2013		 63.48
65.01
Frag5.F2.1
Frag5.R1.1		 GGAACTCCACAYTGGAACAAC
TGCATGTCCACCGCCATCTG		 1658-1678
1994-2013		 60.17
65.01
Frag5.F3.1
Frag5.R1.1		 AGGACAGGCCTTGACTTYTCAG
TGCATGTCCACCGCCATCTG		 1541-1562
1994-2013		 62.94
65.01
Frag5.F4.1
Frag5.R1.1		 GGACAGGCCTTGACTTYTCAG
TGCATGTCCACCGCCATCTG		 1542-1562
1994-2013		 61.81
65.01
Frag5.F5.1
Frag5.R1.1		 AGGACAGGCCTTGACTTYTC
TGCATGTCCACCGCCATCTG		 1541-1560
1994-2013		 60.31
65.01
Frag6.F1.1
Frag6.R1.1		 GAGGCTGAGATGGATGGWGC
TGCATGTCCACCGCCATCTG		 1784-1803
1994-2013		 62.07
65.01
Frag7.F1.1
Frag7.R1.1		 YCAGATGGCGGTGGACATGC
TGAGAACCAGGACATTCCTCCAAAC		 1993-2012
2332-2356		 64.95
64.47
Frag7.F1.1
Frag7.R2.1		 YCAGATGGCGGTGGACATGC
TGTGAGAACCAGGACATTCCTC		 1993-2012
2337-2358		 64.95
61.52
Frag7.F1.1
Frag7.R3.1		 YCAGATGGCGGTGGACATGC
GAGAACCAGGACATTCCTCCAAAC		 1993-2012
2332-2355		 64.95
63.32
Frag7.F1.1
Frag7.R4.1		 YCAGATGGCGGTGGACATGC
TGTGAGAACCAGGACATTCCTCC		 1993-2012
2336-2358		 64.95
63.38
Frag7.F1.1
Frag7.R5.1		 YCAGATGGCGGTGGACATGC
GAACCAGGACATTCCTCCAAAC		 1993-2012
2332-2353		 64.95
61.21
Frag8.F1.1
Frag8.R1.1		 TGTTTGGAGGAATGTCCTGGTTC
GAGTCAGGATGGTACTTGTACC		 2331-2353
2568-2589		 62.55
59.23
Frag8.F2.1
Frag8.R2.1		 TGTTTGGAGGAATGTCCTGGTTCTC
GGAGTCAGGATGGTACTTGTAC		 2331-2355
2569-2590		 64.47
59.57
Frag8.F1.1
Frag8.R3.1		 TGTTTGGAGGAATGTCCTGGTTC
GGGAGTCAGGATGGTACTTGTAC		 2331-2353
2569-2591		 62.55
61.9
Frag8.F4.1
Frag8.R4.1		 TGTTTGGAGGAATGTCCTGG
GGGAGTCAGGATGGTACTTG		 2331-2350
2572-2591		 59.02
59.21