Primer ID (Forward/Reverse) Sequence Start-stop Tm (C)
Frag1.F1.1
Frag1.R1.1		 GATTTGGAAACGAGAGTTTC
TTTATTATTTCCATAGCCTC		 82-101
332-351		 54.16
49.36
Frag1.F3.1
Frag1.R3.1		 GATTTGGAAACGAGAGTTTCTG
TTCCTAGCATTGATWATTCTC		 82-103
382-402		 57.29
52.33
Frag1.F4.1
Frag1.R3.1		 ATTTGGAAACGAGAGTTTCTG
TTCCTAGCATTGATWATTCTC		 83-103
382-402		 55.94
52.33
Frag1.F5.1
Frag1.R3.1		 GGATTTGGAAACGAGAGTTTC
TTCCTAGCATTGATWATTCTC		 81-101
382-402		 56.88
52.33
Frag2.F1.1
Frag2.R1.1		 GATTGTCAATATGCTAAAAC
TTTATTATTTCCATAGCCTC		 142-161
332-351		 49.59
49.36
Frag2.F2.1
Frag2.R1.1		 GGATTGTCAATATGCTAAAAC
TTTATTATTTCCATAGCCTC		 141-161
332-351		 52.52
49.36
Frag2.F3.1
Frag2.R1.1		 GATTGTCAATATGCTAAAACG
TTTATTATTTCCATAGCCTC		 142-162
332-351		 52.59
49.36
Frag2.F4.1
Frag2.R1.1		 ATTGTCAATATGCTAAAACG
TTTATTATTTCCATAGCCTC		 143-162
332-351		 50.98
49.36
Frag2.F5.1
Frag2.R1.1		 TTGTCAATATGCTAAAACGC
TTTATTATTTCCATAGCCTC		 144-163
332-351		 53.69
49.36
Frag3.F2.1
Frag3.R2.1		 GCCAGCCGGACTTCTGCTGGG
GCTGTRGTCAGCAGGAGGCC		 205-225
446-465		 69.79
65.94
Frag3.F3.1
Frag3.R2.1		 TGCCAGCCGGACTTCTGCTG
GCTGTRGTCAGCAGGAGGCC		 204-223
446-465		 67.1
65.94
Frag3.F4.1
Frag3.R2.1		 GCCAGCCGGACTTCTGCTGG
GCTGTRGTCAGCAGGAGGCC		 205-224
446-465		 67.67
65.94
Frag3.F5.1
Frag3.R2.1		 TGCCAGCCGGACTTCTGCTGG
GCTGTRGTCAGCAGGAGGCC		 204-224
446-465		 68.78
65.94