S.No.sgRNA sequencesPAMStrands (+/-)startEndGC (%)Predicted efficiencyGene
4 TATGTATTCAGAGAGACCCG GGG + 346 365 45
70.34
 N
5 ATACCAGTAATGGAGAGGAG AGG + 443 462 45
57.24
 N
12 GTAGAAATAATCTCAGACAT CGG + 857 876 30
54.01
 N
16 GGGGCATTGAATATCAATCG TGG + 1148 1167 45
52.16
 N
20 GTTCTGTGAGCACATCCGGT GGG + 1497 1516 55
53.02
 N
25 GGAGAATCTGAACAAGTTGA GGG + 2565 2584 40
60.28
 P
28 GGGTGGAGTGATTACACATC AGG + 2808 2827 50
51.55
 P
30 GGAGTTAAAGAGCAAAACGT TGG + 3051 3070 40
69.49
 P
34 ACTCCGATGCCAAAGTCCCG AGG + 3585 3604 60
56.47
 P
37 GGATCACCCCCCTACCAAGA AGG + 3702 3721 60
52.74
 P
43 ATATGTTTGTCAATATTAGG GGG - 4500 4519 25
53.35
 P
48 ATACCCCAGGAGCTAACGAG AGG + 5256 5275 55
55.70
 M
49 GTATAGCTCAACACTTCACG TGG - 5352 5371 45
64.14
 M
59 GGGATCAATTAGCCCCCAGA GGG - 6014 6033 55
57.34
 M
60 CTAGTCATAATACATCACAC AGG + 6214 6233 35
60.40
 M
61 GTATTATGCATGAATCTGAA CGG - 6238 6257 30
56.11
 M
75 CTATACTCTCTAAAAGGGAG TGG - 8790 8809 40
60.22
 G
89 GGATGAGGCTAGGATCCTGA GGG + 12314 12333 55
66.82
 L
95 GGGTATAGGGATAGACACGG AGG + 12627 12646 55
59.66
 L
96 GTACTTTCTTCCACGGAACG AGG - 12707 12726 50
55.37
 L
97 GCACTCTTACCAACACCCAG AGG - 12841 12860 55
76.57
 L
98 GCTTGTGTCCTCTTTGACAA AGG - 13141 13160 45
53.07
 L
100 TCTATTTCCTCAGTTCCTCG AGG + 13206 13225 45
51.81
 L
101 CTATAAATCAGACAATACAG AGG + 13481 13500 30
70.77
 L
102 GTGCCTATGAGACAAACACG AGG + 13861 13880 50
68.35
 L
109 GGTTTATTAGATACAACTAA AGG + 14817 14836 25
56.53
 L
111 ATACAACGTCTGTAACCACT GGG - 15496 15515 40
56.20
 L
113 TTAGAAACTGATGATTACAA CGG + 15642 15661 25
53.32
 L
122 GGGGAAATTGAAAGGACTAG TGG + 17099 17118 45
55.29
 L
125 ATATCATAAATAGGACAGCG GGG + 17182 17201 35
59.24
 L