S.No.sgRNA sequencesPAMStrands (+/-)startEndGC (%)Predicted efficiencyGene
1 GGAGTTATCAATCTAAGTTA GGG + 147 166 30
41.24
 N
2 CTAATAGTCCAGAGCTCAGA TGG + 237 256 45
42.57
 N
3 GCTGCCGAGTCAATGAAAGT TGG + 302 321 50
40.73
 N
4 TATGTATTCAGAGAGACCCG GGG + 346 365 45
70.34
 N
5 ATACCAGTAATGGAGAGGAG AGG + 443 462 45
57.24
 N
6 ACTGCTCGAGACAGCAGCAA GGG + 512 531 55
42.45
 N
7 TCATCACTAGTGATTTTAGT TGG - 579 598 30
43.16
 N
8 TACAGCTCCCGACACTGCCG AGG + 652 671 65
45.59
 N
9 GGAAAGTGAAACTAGAAGAT GGG + 673 692 35
45.12
 N
10 TCTTTGCTCTAACTCAGCAA TGG + 729 748 40
47.33
 N
11 ATCCTCATAGAAGTCAAGAA AGG + 812 831 35
40.38
 N
12 GTAGAAATAATCTCAGACAT CGG + 857 876 30
54.01
 N
13 TCTTGAGATTGGGAATCCAG GGG - 875 894 45
41.68
 N
14 TTATCGCGATAGATATTAGA TGG - 943 962 30
49.77
 N
15 ATTATTGTGGAGCTTTGCCA TGG + 1096 1115 40
41.51
 N
16 GGGGCATTGAATATCAATCG TGG + 1148 1167 45
52.16
 N
17 TCAGCAATTGAGTATTCAGA GGG - 1167 1186 35
42.46
 N
18 GAAGAACCTATAGAACAGAG TGG + 1409 1428 40
43.38
 N
19 ACAGTCAGTTACCTTCAAAA GGG + 1435 1454 35
44.26
 N
20 GTTCTGTGAGCACATCCGGT GGG + 1497 1516 55
53.02
 N
21 ACTATCATAATACGACACTA AGG + 1759 1778 30
40.48
 N
22 GGGTTTAGATCTATTATCCA AGG - 1767 1786 35
48.35
 N
23 CTGTTCGGCGATTAAAAAGG GGG - 1880 1899 45
42.36
 N
24 TGCAATCTATTTCTGAAACG GGG - 2431 2450 35
48.29
 P
25 GGAGAATCTGAACAAGTTGA GGG + 2565 2584 40
60.28
 P
26 TGACATACAACTGGACCCAG TGG + 2714 2733 50
43.09
 P
27 GGATTTATCTCTCAATCTAT GGG - 2777 2796 30
44.85
 P
28 GGGTGGAGTGATTACACATC AGG + 2808 2827 50
51.55
 P
29 ACACTCTTCATTGTCCCCAT AGG - 2894 2913 45
44.23
 P
30 GGAGTTAAAGAGCAAAACGT TGG + 3051 3070 40
69.49
 P
31 GTACACATCAGATGACGAAG AGG + 3116 3135 45
40.71
 P
32 GATAATCTAGAAACTAACTT TGG - 3348 3367 25
42.71
 P
33 GATAAAACTGAGATCGTCAA CGG + 3528 3547 35
48.55
 P
34 ACTCCGATGCCAAAGTCCCG AGG + 3585 3604 60
56.47
 P
35 GGTCGAAGAGTGGTGCAACC CGG + 3670 3689 60
44.11
 P
36 GGTGCAACCCGGCATGTTCG AGG + 3681 3700 65
44.79
 P
37 GGATCACCCCCCTACCAAGA AGG + 3702 3721 60
52.74
 P
38 CCATGATGATAATGATACCA GGG + 4123 4142 35
42.79
 P
39 TCGTTGACAAACGAACCGTA TGG + 4266 4285 45
43.41
 P
40 ATAGGCACTCAAGAATAAAA AGG - 4379 4398 30
49.88
 P
41 AGGCACTCAAGAATAAAAAG GGG - 4381 4400 35
44.32
 P
42 GATTGGTTACCTGAATAAAG CGG + 4448 4467 35
48.37
 P
43 ATATGTTTGTCAATATTAGG GGG - 4500 4519 25
53.35
 P
44 TATGTTTGTCAATATTAGGG GGG - 4501 4520 30
40.84
 P
45 CTAAACATAATAACATAAAA TGG + 4901 4920 15
45.80
 P
46 TTACCCTGACTGTTCCCTCG AGG - 5004 5023 55
42.43
 M
47 TTCATCCTCGAGATAAGCAC CGG - 5237 5256 45
43.59
 M
48 ATACCCCAGGAGCTAACGAG AGG + 5256 5275 55
55.70
 M
49 GTATAGCTCAACACTTCACG TGG - 5352 5371 45
64.14
 M
50 TATAGCTCAACACTTCACGT GGG - 5353 5372 40
44.15
 M
51 TCATCCCCAAGATCTTCTGG AGG + 5410 5429 50
43.34
 M
52 CTCTAAATCACCTCGTTCCG TGG + 5508 5527 50
42.73
 M
53 ATATGTTTAGATGCATGTGC GGG - 5553 5572 35
44.49
 M
54 TATGTTTAGATGCATGTGCG GGG - 5554 5573 40
40.06
 M
55 GGGGAGGTAAAGAGGAGGCC AGG - 5573 5592 65
40.81
 M
56 TATATTATCGATATTTAGAA TGG - 5786 5805 15
42.91
 M
57 GCCTCCTTCATGCTACACTT GGG + 5804 5823 50
42.47
 M
58 GGAACTTTGTCCGTCGTGCA GGG + 5826 5845 55
45.85
 M
59 GGGATCAATTAGCCCCCAGA GGG - 6014 6033 55
57.34
 M
60 CTAGTCATAATACATCACAC AGG + 6214 6233 35
60.40
 M
61 GTATTATGCATGAATCTGAA CGG - 6238 6257 30
56.11
 M
62 GCTATATCTCAGGCATTCGG TGG + 7341 7360 50
47.38
 F
63 TCAACAATGATAATTCAGAA TGG + 7555 7574 25
48.66
 F
64 GATTTTGCCTAATTACAAAG AGG + 7639 7658 30
47.90
 F
65 TACGCCAATTGACAACCAGA GGG - 7864 7883 45
47.17
 F
66 TTGATGCCTGAAGGTCCATA CGG - 8235 8254 45
42.19
 F
67 AGAGTCAGACCTACAAGCAG TGG + 8247 8266 50
42.73
 F
68 TAGATCATCCTGAATAATCG AGG + 8372 8391 35
40.92
 F
69 TGTTGTATACAGCACTACAG TGG - 8443 8462 40
48.15
 F
70 ACATTGGTCATAAATAAAGA AGG - 8496 8515 25
41.18
 F
71 TGACAAATAAGGATTCAAAT TGG + 8564 8583 25
44.26
 F
72 ATGATCCGATTCTTTCCAGG TGG - 8587 8606 45
48.73
 F
73 GGTACCACTTTGACCGACTA CGG - 8660 8679 50
40.37
 F
74 TCTAAGCTATCACATATCAA AGG + 8752 8771 30
43.07
 G
75 CTATACTCTCTAAAAGGGAG TGG - 8790 8809 40
60.22
 G
76 TAAGAGCTACTACGGAACCA TGG + 9017 9036 45
41.01
 G
77 GGTATCCAACAGCAGATCAA AGG + 9207 9226 45
44.63
 G
78 CCCATTGCTGGCTATGGACG AGG + 9599 9618 60
43.04
 G
79 CTATTTTGCATATAGCCACC TGG + 9623 9642 40
45.52
 G
80 GTTGGAGAGGTACTAGACAG AGG + 9696 9715 50
45.00
 G
81 GGACCTTCAGGCATCAAACA GGG + 9996 10015 50
43.20
 G
82 ACTTTATCTGTAAAGACTGG AGG - 10233 10252 35
47.34
 G
83 TTATCATTGTTGAAGCTCAC TGG - 10680 10699 35
42.87
 G
84 ATACAAAAAGCAAATCAGAG AGG - 10954 10973 30
40.72
 G
85 ATATTAAGACAATCCAAACA GGG + 11598 11617 25
43.25
 L
86 CTATTCTCCATCACATCCCA AGG + 11694 11713 45
45.86
 L
87 AATGTATTGTGATGTCCTAG AGG + 12119 12138 35
43.85
 L
88 TGTCCATCAAAGAGAAACAA AGG - 12227 12246 35
41.34
 L
89 GGATGAGGCTAGGATCCTGA GGG + 12314 12333 55
66.82
 L
90 GATGAGGCTAGGATCCTGAG GGG + 12315 12334 55
44.60
 L
91 AGTAATACTTCCCTGCACGA CGG - 12389 12408 45
40.52
 L
92 GTTTCTCCTGAACTCAAGCA TGG - 12543 12562 45
47.68
 L
93 TCTGCGGGACTATAATAAAT GGG + 12607 12626 35
40.12
 L
94 GGACTATAATAAATGGGTAT AGG + 12613 12632 30
49.75
 L
95 GGGTATAGGGATAGACACGG AGG + 12627 12646 55
59.66
 L
96 GTACTTTCTTCCACGGAACG AGG - 12707 12726 50
55.37
 L
97 GCACTCTTACCAACACCCAG AGG - 12841 12860 55
76.57
 L
98 GCTTGTGTCCTCTTTGACAA AGG - 13141 13160 45
53.07
 L
99 ATATTTTAAGGAGAACGGGA TGG + 13142 13161 35
40.63
 L
100 TCTATTTCCTCAGTTCCTCG AGG + 13206 13225 45
51.81
 L
101 CTATAAATCAGACAATACAG AGG + 13481 13500 30
70.77
 L
102 GTGCCTATGAGACAAACACG AGG + 13861 13880 50
68.35
 L
103 TTAGAACCGACTCTTCACAC AGG - 14290 14309 45
44.49
 L
104 GCATTAATCCCGGCACCTAT TGG + 14379 14398 50
44.84
 L
105 GTGAGTCGTTGTCGTCTACG GGG - 14425 14444 55
44.94
 L
106 GGGGTGATCCTCGAACATGC CGG - 14536 14555 60
45.28
 L
107 GGGTGATCCTCGAACATGCC GGG - 14537 14556 60
44.61
 L
108 GGTGCCAGAGAGGAAATTGC TGG + 14796 14815 55
43.11
 L
109 GGTTTATTAGATACAACTAA AGG + 14817 14836 25
56.53
 L
110 ATAGGTAGCCTCTTCTGGGG TGG - 14862 14881 55
44.90
 L
111 ATACAACGTCTGTAACCACT GGG - 15496 15515 40
56.20
 L
112 TTAGGGTTATCGGTATTGGA AGG + 15603 15622 40
44.85
 L
113 TTAGAAACTGATGATTACAA CGG + 15642 15661 25
53.32
 L
114 CGTTCTTGAGATTATAACAA AGG + 15917 15936 30
44.43
 L
115 GGGCCTTTGAAATTCATCAT AGG + 16072 16091 40
46.68
 L
116 ATTCATCATAGGCGTCCTAG AGG + 16083 16102 45
42.80
 L
117 GGCTTGCTATTGCCTACACA GGG + 16224 16243 50
47.66
 L
118 GGAGCTCAAAGGTTGTTTAT AGG + 16917 16936 40
44.46
 L
119 GTTATTATATCAGTCTACAT TGG + 16961 16980 25
43.15
 L
120 ATAGATGGAGATTATATACC AGG + 17013 17032 30
44.13
 L
121 TGGCCTAGTCTGAAATACAT AGG - 17044 17063 40
47.42
 L
122 GGGGAAATTGAAAGGACTAG TGG + 17099 17118 45
55.29
 L
123 CTAGTGGTGCCCCTATTCAA TGG + 17115 17134 50
46.88
 L
124 GTATATCATAAATAGGACAG CGG + 17180 17199 30
46.64
 L
125 ATATCATAAATAGGACAGCG GGG + 17182 17201 35
59.24
 L
126 GGTGATAACTGCAGAGACCA GGG - 17634 17653 50
46.28
 L