S.No.	sgRNA sequences	PAM	Strands (+/-)	start	End	GC (%)	Predicted efficiency
1	GGAGGTGAGAGGATACACAA	AGG	+	7948	7967	50	85.423087
2	GGTCACGACGTAACGACGCG	TGG	-	1910	1929	65	74.858481
3	GGAGGTATGTACATGAGTAG	AGG	-	4771	4790	45	72.283255
4	GGACTGATACTCGAACCAAA	GGG	-	322	341	45	68.954579
5	GGTTCAACTACAGCACAACG	TGG	-	10098	10117	50	68.122062
6	GGCCCTGGAGGGAAACACAT	AGG	-	2815	2834	60	67.994919
7	GGTAAGATCCTATTGCTACG	AGG	+	1141	1160	45	67.797992
8	GGATTGGGAAGCTGAGGTGG	AGG	-	1606	1625	60	67.047518
9	GGGGTCTTGCTGCTGCCAAA	CGG	-	9054	9073	60	66.55558
10	GGAGGACATGCCGTATCCCG	GGG	+	7805	7824	65	66.092378
11	GGGGCCCTATGCCGTACAGG	AGG	-	2968	2987	70	65.787234
12	GGGTTCCGTGGGGAAAAAAG	AGG	+	313	332	55	65.450271
13	GTACGGATGCAACGTCAGCG	AGG	-	2534	2553	60	65.020911
14	GGGAGAATGGATGACCACTG	AGG	+	10060	10079	55	63.429789
15	ATACACATACCAAAACAAAG	TGG	+	9367	9386	30	63.274327
16	GGAGAGTTCGGGAAAGCAAA	AGG	+	9044	9063	50	63.066434
17	CGTATTACCAGAGATCCCAG	AGG	-	4175	4194	50	62.909525
18	TCTACGTTGGACTATCCGAT	CGG	-	4542	4561	45	62.339016
19	GGGTCACTCAACTTGTGTGG	GGG	-	8506	8525	55	62.30956
20	GGGTCTCAGAGAGGGCAGGG	TGG	-	3115	3134	70	62.037519
21	GGTAGACTAGAAACGTGAAG	GGG	-	2949	2968	45	61.882592
22	AGTGGCGAGGAGTCACGCCG	AGG	-	6010	6029	70	60.980758
23	GGATAGACTCAAACGAATGG	CGG	+	9616	9635	45	60.053243

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