S.No.	sgRNA sequences	PAM	Strands (+/-)	start	End	GC (%)	Predicted efficiency
1	GGAGGTGAGAGGATACACAA	AGG	+	7948	7967	50	85.423087
2	GGTCACGACGTAACGACGCG	TGG	-	1910	1929	65	74.858481
3	GGAGGTATGTACATGAGTAG	AGG	-	4771	4790	45	72.283255
4	GGACTGATACTCGAACCAAA	GGG	-	322	341	45	68.954579
5	GGTTCAACTACAGCACAACG	TGG	-	10098	10117	50	68.122062
6	GGCCCTGGAGGGAAACACAT	AGG	-	2815	2834	60	67.994919
7	GGTAAGATCCTATTGCTACG	AGG	+	1141	1160	45	67.797992
8	GGATTGGGAAGCTGAGGTGG	AGG	-	1606	1625	60	67.047518
9	GGGGTCTTGCTGCTGCCAAA	CGG	-	9054	9073	60	66.55558
10	GGAGGACATGCCGTATCCCG	GGG	+	7805	7824	65	66.092378
11	GGGGCCCTATGCCGTACAGG	AGG	-	2968	2987	70	65.787234
12	GGGTTCCGTGGGGAAAAAAG	AGG	+	313	332	55	65.450271
13	GTACGGATGCAACGTCAGCG	AGG	-	2534	2553	60	65.020911
14	GGGAGAATGGATGACCACTG	AGG	+	10060	10079	55	63.429789
15	ATACACATACCAAAACAAAG	TGG	+	9367	9386	30	63.274327
16	GGAGAGTTCGGGAAAGCAAA	AGG	+	9044	9063	50	63.066434
17	CGTATTACCAGAGATCCCAG	AGG	-	4175	4194	50	62.909525
18	TCTACGTTGGACTATCCGAT	CGG	-	4542	4561	45	62.339016
19	GGGTCACTCAACTTGTGTGG	GGG	-	8506	8525	55	62.30956
20	GGGTCTCAGAGAGGGCAGGG	TGG	-	3115	3134	70	62.037519
21	GGTAGACTAGAAACGTGAAG	GGG	-	2949	2968	45	61.882592
22	AGTGGCGAGGAGTCACGCCG	AGG	-	6010	6029	70	60.980758
23	GGATAGACTCAAACGAATGG	CGG	+	9616	9635	45	60.053243
24	GGAGCTCAATGCTATCCTAG	AGG	+	2695	2714	50	59.823257
25	GCGTCAGTAGAGGTGACTGG	CGG	-	1139	1158	60	59.453613
26	GGAAGAGAAAACTCAGGAGG	TGG	+	9152	9171	50	59.378306
27	GGAGTGGGAGTCATGCAAGA	GGG	+	4709	4728	55	58.512124
28	GGAAAGTGAACCCAAGACAT	TGG	-	7522	7541	45	58.506302
29	GGAGGATGTGAACCTCGGCT	CGG	+	8416	8435	60	58.085193
30	GGGGGCACGGTGTAGAGCAA	AGG	-	8264	8283	65	57.809633
31	GGAGGTGGAGTCGAAGGGTT	AGG	+	9167	9186	60	57.676634
32	GGGTGTGCAGAGACTGATGA	AGG	+	6026	6045	55	57.143426
33	CCTGTGAATGAGCTGCCCCA	TGG	+	2795	2814	60	57.016355
34	GGAGATGAGTACATGTATGG	AGG	+	6002	6021	45	57.004869
35	GGGTCATACCTAGTTCAGAA	GGG	-	5987	6006	45	56.750766
36	GGAGCATCGTTATCCTAGAA	TGG	-	9632	9651	45	56.736394
37	GGAAACACATAGGAGAGTGG	AGG	-	2825	2844	50	56.369272
38	GTACTGGTCTTTGAGAGCAA	AGG	-	10686	10705	45	56.295554
39	GGATACTGTTACATTCTTAG	GGG	-	8662	8681	35	56.151602
40	GTACTCAAGGTGCTTCCAGA	AGG	-	4587	4606	50	55.695447
41	GGAGGTGCAGAGAATTGGTC	CGG	-	3136	3155	55	55.641512
42	GGTGGAGGACTCAAAAGAGA	AGG	-	1621	1640	50	55.585225
43	GGGTGAGGTTGGACACAACT	TGG	-	6079	6098	55	55.382785
44	GGTTACAGAACCCAAGTGAA	AGG	+	3251	3270	45	55.011059
45	GTACTTTGGTGAGAAACACG	TGG	-	9173	9192	45	55.001001
46	GTAGGAACTCCCGTGCCGCT	CGG	-	3002	3021	65	54.979493
47	TCTCGCTCCGATACTCCGGT	AGG	-	4673	4692	60	54.889416
48	GGATTGGTGTGATCGTCGCA	CGG	-	8404	8423	55	54.759223
49	GGAAGACTTGATCCATACTG	GGG	+	4787	4806	45	54.620585
50	GATTGAGGAGAACGACCATA	TGG	+	10111	10130	45	54.476314
51	GTAGTGGATACTAGGACGCG	TGG	-	506	525	55	54.354705
52	GGAAGTAGTCAGAGACGTGT	GGG	-	4747	4766	50	54.309189
53	ACAAGACTCCTGTAACAAAA	TGG	+	10137	10156	35	54.224111
54	ATCTCCCCAAGATAACCAGA	TGG	+	6832	6851	45	54.117302
55	GCTGTAGTAGACCAAGGTGA	GGG	-	10127	10146	50	54.100516
56	ATAATGATGTTGAAGCCTGG	AGG	+	2541	2560	40	54.028563
57	GGTTGTGGACTTTTTGGCAA	AGG	+	1286	1305	45	53.869725
58	ATTGTCAATATGCTAAAACG	CGG	+	143	162	30	53.660585
59	GGGTCATGGACCCATCAGAA	TGG	+	223	242	55	53.30486
60	CTACTGTTACTCACAAGGAG	TGG	+	4181	4200	45	53.294035
61	TGAGAGGACCAAAAAGGCCG	AGG	-	5584	5603	55	53.088693
62	ATACTGACCAAAATCCGTAG	AGG	-	4893	4912	40	52.949603
63	CTTGCCGAGGAAGGACCGAG	GGG	-	9035	9054	65	52.874043
64	GGAGTCCCGCTGCTAATGAT	GGG	+	7175	7194	55	52.615437
65	GGAATATTCAAGACAAAGGA	CGG	+	4940	4959	35	52.579877
66	GGGTAGTAATCGTCGCCCTG	AGG	-	3598	3617	60	52.563763
67	TCAGGATTCGAAGGTTTCGG	AGG	-	9268	9287	50	52.496096
68	TCTGCTCGAAAGGTGAAGAG	AGG	-	3094	3113	50	52.0763
69	CTAAAGTTCGAGAAGTGACA	CGG	-	7494	7513	40	52.065173
70	TAGTCAGGCCGAGAACGCCA	TGG	+	10466	10485	60	51.979161
71	TCAACAGATGGGGTTCCGTG	GGG	+	303	322	55	51.97864
72	GGAACTCCCGTGCCGCTCGG	AGG	-	3005	3024	75	51.88836
73	GGAAACGAGATGTGGCACGG	GGG	+	2509	2528	60	51.812689
74	GCAGCAGAGATCACTAGACG	CGG	+	470	489	55	51.573214
75	TCCACACTGGAACAACAAAG	AGG	+	1663	1682	45	51.558698
76	GGAAGGGCCGTAGCGAGGAT	AGG	-	10259	10278	65	51.436722
77	GGGTTAGGATTGCAAAGACT	TGG	+	9182	9201	45	51.403111
78	GCTCCCAAAGAAGTGAAGAA	AGG	+	4625	4644	45	51.371396
79	GGAAAAAGAGAAAAGAAGCA	AGG	+	9023	9042	35	51.229152
80	GGTACAAGGCCTATTCGACG	TGG	-	1265	1284	55	51.206433
81	ACTGGCTTGAAAAAAGACCA	GGG	+	8163	8182	40	50.888716
82	AATATTCAAGACAAAGGACG	GGG	+	4942	4961	35	50.871649
83	CCTCCTGCTGACTACAGCCA	TGG	+	448	467	60	50.581067
84	GGGGTCATACCTAGTTCAGA	AGG	-	5986	6005	50	50.462681
85	GGGTCAGAAGTGTATGTGTA	TGG	-	6201	6220	45	50.297819
86	TTAAGCTGAGGACAGAGCAA	AGG	+	6165	6184	45	50.256125
87	GTAGTAATATCTACTTATGG	GGG	-	8246	8265	30	50.233415